使用纤维素-钛白粉复合扩张床阴离子吸附剂提取D-2-氯丙酸脱卤酶

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使用纤维素-钛白粉复合扩张床阴离子吸附剂提取D-2-氯丙酸脱卤酶

雷引林^a; 金志华^a,b; 童微星^a; 姚善泾^a,b; 朱自强^b

^a Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China
^b Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-08-28 发布日期:2006-08-28
通讯作者: 雷引林

Expanded Bed Recovery of D-2-Chloropropionic Acid Dehalogenase Using TiO2-Densified Cellulose Anion Exchanger

LEIYinlin^a;JINZhihua^a,b; TONGWeixing^a; YAOShanjing^a,b; ZHUZiqiang^b

^a Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China
^b Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-08-28 Published:2006-08-28
Contact: LEI Yinlin

摘要/Abstract

摘要： The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U•ml－1 adsorbent, and two active peaks were eluted re-spectively at 0.15mol•L－1 and 0.3mol•L－1 (NH4)2SO4. The result indicated that the overall enzyme yield was 68%, with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.

关键词: expanded bed adsorption;2-chloropropionic acid;dehalogenase;cellulose;ion exchange

Abstract: The TiO2-densified cellulose composite beads were activated by epichlorohydrin and coupled with diethylamine, to function as an anion exchanger for expanded bed chromatography. The adsorbent exhibited a favorable performance of expanded bed adsorption for proteins, and therefore was applied to the expanded bed recovery of D-2-chloropropionic acid dehalogenase directly from the unclarified homogenate of Pseudomonas sp. NT21. The binding capacity of the dehalogenase was found to be 8.54U•ml－1 adsorbent, and two active peaks were eluted re-spectively at 0.15mol•L－1 and 0.3mol•L－1 (NH4)2SO4. The result indicated that the overall enzyme yield was 68%, with a purification factor of 22. In comparison to other recovery processes, the yield of the expanded bed process rises at least 70%, simultaneously saving a great deal of operation time and costs.

Key words: expanded bed adsorption, 2-chloropropionic acid, dehalogenase, cellulose, ion exchange

雷引林, 金志华, 童微星, 姚善泾, 朱自强. 使用纤维素-钛白粉复合扩张床阴离子吸附剂提取D-2-氯丙酸脱卤酶[J]. CIESC Journal.

LEIYinlin,JINZhihua, TONGWeixing, YAOShanjing, ZHUZiqiang. Expanded Bed Recovery of D-2-Chloropropionic Acid Dehalogenase Using TiO2-Densified Cellulose Anion Exchanger[J]. .

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使用纤维素-钛白粉复合扩张床阴离子吸附剂提取D-2-氯丙酸脱卤酶

Expanded Bed Recovery of D-2-Chloropropionic Acid Dehalogenase Using TiO2-Densified Cellulose Anion Exchanger

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