金志华a,b; 程休b; 岑沛霖b
JINZhihuaa,b; CHENGXiub; CENPeilinb
摘要: The effects of glucose and inorganic phosphate on mycelium growth and spinosad production with Saccharopolyspora spinosa were studied. The results showed that the increase of glucose concentration from 18.6g•L-1 to 58.8g•L-1 could promote the mycelium growth and spinosad production. And when the glucose con-centration increased from 58.8g•L-1 to 79.6g•L-1, no obvious change was detected but a slight drop in spinosad production was observed, whereas, when the glucose concentration increased from 79.6g•L-1 to 115.3g•L-1, sub-stantial decrease in both mycelium growth and spinosad production occurred. The increase of phosphate concentra-tion from 3.68mmol•L-1 to 29.41mmol•L-1 rendered corresponding increase in mycelium growth and spinosad production. When phosphate concentration increased from 29.41mmol•L-1 to 44.12mmol•L-1, mycelium growth slightly increased and spinosad production dropped, while when phosphate concentration increased from 44.12mmol•L-1 to 57.62mmol•L-1, both mycelium growth and spinosad production decreased sharply. Conclusively, the optimal initial concentration of glucose and phosphate is 58.8g•L-1 and 29.41mmol•L-1, respectively. The spinosad fermentation in the production medium containing 58.8g•L-1 glucose and 29.41mmol•L-1 phosphate was scaled up in 5-L fermentation and the spinosad production reached 507mg•L-1, which was 28% higher than that in the flask fermentation.