白假丝酵母PDY-07厌氧生物降解4-氯酚的研究

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白假丝酵母PDY-07厌氧生物降解4-氯酚的研究

闻建平^a; 李红梅^a,c; 白静^a; 姜岩^a,b

^a Department of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
^b School of Life Sciences and Chemisty, Harbin College, Harbin 150016, China
^c Yancheng Textile Vocational and Technical College, Yancheng 224005, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-12-28 发布日期:2006-12-28
通讯作者: 闻建平

Biodegradation of 4-chlorophenol by Candida albicans PDY-07 under anaerobic conditions

WEN Jianping^a; LI Hongmei^a,c; BAI Jing^a; JIANG Yan^a,b

^aDepartment of Biochemical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
^b School of Life Sciences and Chemisty, Harbin College, Harbin 150016, China
^c Yancheng Textile Vocational and Technical College, Yancheng 224005, China

Received:1900-01-01 Revised:1900-01-01 Online:2006-12-28 Published:2006-12-28
Contact: WEN Jianping

摘要/Abstract

摘要： Candida albicans PDY-07 was isolated from activated sludge under anaerobic conditions and identified as a member belonging to the genus Candida. Pure culture of C. albicans PDY-07, biodegradation of 4-chlorophenol (4-CP) was carried out under anaerobic conditions in Erlenmeyer flasks at 35℃, with an initial pH of 7.0—7.2 and a starting inoculum of 10% (by volume). The results showed that, under the above-mentioned conditions, C. albicans PDY-07 could thoroughly biodegrade 4-CP up to a concentration of 300mg•L－1 within 244h and that it had a high tolerance potential of up to 440mg•L－1 for 4-CP. With the increase in the initial concentrations of 4-CP, substrate inhibition was obviously enhanced. There was increased consumption of 4-CP, which was not assimilated by the cell for growth but was used to counteract the strong substrate inhibition. In addition, the cell growth and substrate-degradation kinetics of 4-CP as the sole source of carbon and energy for the strain in batch cultures were also investigated over a wide range of substrate concentrations (2.2—350mg•L－1), using the proposed cell growth and degradation kinetic models. The results recorded from these experiments showed that the proposed kinetic models adequately described the dynamic behavior of 4-CP biodegradation by C. albicans PDY-07.

关键词: Candida albicans;4-chlorophenol biodegradation;anaerobic;substrate inhibition

Abstract: Candida albicans PDY-07 was isolated from activated sludge under anaerobic conditions and identified as a member belonging to the genus Candida. Pure culture of C. albicans PDY-07, biodegradation of 4-chlorophenol (4-CP) was carried out under anaerobic conditions in Erlenmeyer flasks at 35℃, with an initial pH of 7.0—7.2 and a starting inoculum of 10% (by volume). The results showed that, under the above-mentioned conditions, C. albicans PDY-07 could thoroughly biodegrade 4-CP up to a concentration of 300mg•L－1 within 244h and that it had a high tolerance potential of up to 440mg•L－1 for 4-CP. With the increase in the initial concentrations of 4-CP, substrate inhibition was obviously enhanced. There was increased consumption of 4-CP, which was not assimilated by the cell for growth but was used to counteract the strong substrate inhibition. In addition, the cell growth and substrate-degradation kinetics of 4-CP as the sole source of carbon and energy for the strain in batch cultures were also investigated over a wide range of substrate concentrations (2.2—350mg•L－1), using the proposed cell growth and degradation kinetic models. The results recorded from these experiments showed that the proposed kinetic models adequately described the dynamic behavior of 4-CP biodegradation by C. albicans PDY-07.

Key words: Candida albicans, 4-chlorophenol biodegradation, anaerobic, substrate inhibition

闻建平, 李红梅, 白静, 姜岩. 白假丝酵母PDY-07厌氧生物降解4-氯酚的研究[J]. CIESC Journal.

WEN ,Jianping, LI ,Hongmei, BAI ,Jing, JIANG ,Yan. Biodegradation of 4-chlorophenol by Candida albicans PDY-07 under anaerobic conditions[J]. .

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白假丝酵母PDY-07厌氧生物降解4-氯酚的研究

Biodegradation of 4-chlorophenol by Candida albicans PDY-07 under anaerobic conditions

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