CIESC Journal
• 生物化学工程、制药、食品和天然产物加工 • 上一篇 下一篇
胡健;徐岩
江南大学生物工程学院,教育部工业生物技术重点实验室,江苏 无锡 214036
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Strains screening for asymmetric reduction of 2-octanone in aqueous-organic solvents biphasic systems
HU Jian;XU Yan
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摘要: 从93株酵母、霉菌和细菌中筛选出能不对称还原2-辛酮的菌株.经过筛选获得一株博伊丁假丝酵母Candida boidinii CECT 10035.该菌种在底物浓度为78 mmol·L-1时,能转化得到S-2-辛醇,2-辛酮转化率为32.6%,e.e.值达到95.6%.同时,还筛选出一株酒明串珠菌Oenococcus oeni CECT 4730,可以转化得到R-2-辛醇,当底物浓度为78 mmol·L-1时,2-辛酮转化率为99.8%,e.e.值为97.8%.
Abstract: Asymmetric reduction of 2-octanone by yeast,mold and bacteria in aqueous-organic solvents biphasic systems were investigated.Candida boidinii CECT 10035 catalyzed the reduction of 2-octanone to S-2-octanol with conversion of 32.6% and optical purity of 95.6%.Different from the yeast strain, Oenococcus oeni CECT 4730 catalyzed the reduction of 2-octanone to R-2-octanol with higher activity and optical purity.When the concentration of 2-octanone was 78 mmol·L-1,the conversion and enantiomeric excess were 99.8% and 97.8%.
胡健;徐岩 . 两相系统中不对称还原2-辛酮的菌株筛选 [J]. CIESC Journal.
HU Jian;XU Yan.
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https://hgxb.cip.com.cn/CN/Y2006/V57/I10/2383