CIESC Journal
• 生物化学工程、制药、食品和天然产物加工 • 上一篇 下一篇
徐坚;杨立明;王玉军;骆广生;戴猷元
清华大学化学工程系,化学工程联合国家重点实验室,北京 100084
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Improvement of lipase immobilization by surface modification of SBA-15 mesoporous silica
XU Jian;YANG Liming;WANG Yujun;LUO Guangsheng;DAI Youyuan
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Abstract: Three kinds of support for enzyme immobilization were prepared by modifying SBA-15 mesoporous silica with thiol,methyl and hexyl groups respectively.The prepared supports were characterized with FT-IR and BET method and exhibited high specific surface area and uniform pore size.Furthermore,unmodified SBA-15 and the modified SBA-15 materials were used for lipase immobilization.The results were compared in terms of enzyme adsorption capacity,activity,activity retention and stability.The modified SBA-15 materials with thiols(SBA-15-PrSH)showed good performance for lipase immobilization.Compared with unmodified SBA-15,the adsorption capacity of SBA-15-PrSH to lipase was improved by more than 80%,and the activity retention increased by more than 10%.
徐坚;杨立明;王玉军;骆广生;戴猷元 . 介孔分子筛SBA-15表面改性对脂肪酶固定化的强化作用 [J]. CIESC Journal.
XU Jian;YANG Liming;WANG Yujun;LUO Guangsheng;DAI Youyuan.
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https://hgxb.cip.com.cn/CN/Y2006/V57/I10/2407