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于少明;程俊;左鹏;陈天虎;史铁钧
合肥工业大学化学工程学院;资源与环境工程学院,安徽 合肥 230009
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Immobilization of horseradish peroxidase to pillared clay as carrier
YU Shaoming;CHENG Jun;ZUO Peng;CHEN Tianhu;SHI Tiejun
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Abstract:
Aluminum pillared interlayered clay (Al-PILC) calcined at 773 K was the most appropriate support for immobilization of horseradish peroxidase (HRP) among several materials investigated.HRP could be immobilized on the Al-PILC via hydrogen bonding between functional groups of support and amino groups of the enzyme. The support and immobilized enzyme were characterized with N2-adsorption and desorption, XRD and FT-IR.An optimum condition for immobilization with enzyme immobilization efficiency of about 100% was obtained when the dose of support was greater than 0.45 mg per unit enzyme activity and reaction time exceeded 15 min.HRP immobilized on Al-PILC exhibited good catalytic performance.
于少明;程俊;左鹏;陈天虎;史铁钧. 以层柱黏土为载体固定辣根过氧化物酶 [J]. CIESC Journal.
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https://hgxb.cip.com.cn/CN/Y2006/V57/I12/3021