Abstract: The recent progress in complex biocatalysts assembled by biocompatible inorganic/metal nanoparticles and enzymes is reviewed.The assembly methods and the effects of nanoparticles on the catalytic activity and efficiency of enzymes are analyzed by taking account of the properties of nano-materials.Furthermore，several nanoparticle-carrier effects on enzymes are summarized.Finally，the status of converting native enzymes to highly efficient conjugating biocatalysts by assembling enzymes and inorganic/metal nanoparticles is discussed，and the prospective application is also forecasted.