丙酸盐影响利迪链霉菌AS 4.2501生产利迪链菌素及其碳流分布

CIESC Journal

• BIOTECHNOLOGY & BIOENGINEERING • 下一篇

丙酸盐影响利迪链霉菌AS 4.2501生产利迪链菌素及其碳流分布

李良智; 郑卉; 元英进

Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-04-28 发布日期:2007-04-28
通讯作者: 李良智

Effects of propionate on streptolydigin production and carbon flux distribution in
Streptomyces lydicus AS 4.2501

LILiangzhi;ZHENGHui; YUANYingjin

Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

Received:1900-01-01 Revised:1900-01-01 Online:2007-04-28 Published:2007-04-28
Contact: LI Liangzhi

摘要/Abstract

摘要： To achieve higher antibiotic streptolydigin productivity through metabolic regulation, propionate was fed during the fermentation of Streptomyces lydicus AS 4.2501. The effects of propionate feeding on streptolydigin production and intracellular fluxes were investigated. The highest streptolydigin production yield of 95.10mg•L－1 was obtained when 2mmol•L－1 of sodium propionate was added at 60h of cultivation into shake-flask culture. This yield is 23.06% higher when compared to that of a batch culture without propionate supplementation. It was also found that when propionate was added, much more organic acids were excreted. Metabolic flux analysis was performed and it demonstrated that the carbon fluxes of the pentose phosphate pathway and the anaplerotic reaction were significantly increased after propionate feeding. The carbon flux from pyruvate to acetyl-CoA was determined to be 24.7, which was 12.27% higher than that in the batch culture. This study indicated that the glucose-6-phosphate and pyruvate nodes were potential bottlenecks for increasing streptolydigin productivity. Potential targets and strategies that could be manipulated through genetic and process engineering to increase the production of streptolydigin were also suggested.

关键词: streptolydigin;propionate;feeding;metabolic flux;Streptomyces lydicus;antibiotic

Abstract: To achieve higher antibiotic streptolydigin productivity through metabolic regulation, propionate was fed during the fermentation of Streptomyces lydicus AS 4.2501. The effects of propionate feeding on streptolydigin production and intracellular fluxes were investigated. The highest streptolydigin production yield of 95.10mg•L－1 was obtained when 2mmol•L－1 of sodium propionate was added at 60h of cultivation into shake-flask culture. This yield is 23.06% higher when compared to that of a batch culture without propionate supplementation. It was also found that when propionate was added, much more organic acids were excreted. Metabolic flux analysis was performed and it demonstrated that the carbon fluxes of the pentose phosphate pathway and the anaplerotic reaction were significantly increased after propionate feeding. The carbon flux from pyruvate to acetyl-CoA was determined to be 24.7, which was 12.27% higher than that in the batch culture. This study indicated that the glucose-6-phosphate and pyruvate nodes were potential bottlenecks for increasing streptolydigin productivity. Potential targets and strategies that could be manipulated through genetic and process engineering to increase the production of streptolydigin were also suggested.

Key words: streptolydigin, propionate, feeding, metabolic flux, Streptomyces lydicus, antibiotic

李良智, 郑卉, 元英进. 丙酸盐影响利迪链霉菌AS 4.2501生产利迪链菌素及其碳流分布[J]. CIESC Journal.

LILiangzhi,ZHENGHui, YUANYingjin. Effects of propionate on streptolydigin production and carbon flux distribution in
Streptomyces lydicus AS 4.2501[J]. .

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丙酸盐影响利迪链霉菌AS 4.2501生产利迪链菌素及其碳流分布

Effects of propionate on streptolydigin production and carbon flux distribution in
Streptomyces lydicus AS 4.2501

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丙酸盐影响利迪链霉菌AS 4.2501生产利迪链菌素及其碳流分布

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Effects of propionate on streptolydigin production and carbon flux distribution in
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