CIESC Journal

• BIOTECHNOLOGY & BIOENGINEERING • 上一篇    下一篇

Ralstonia eutropha PHB4重组菌合成PHA共聚物及性质测定

陈晶瑜a; 张磊a; 陈金春a; 陈国强a,b   

  1. a MOE Laboratory of Protein Science, Department of Biological Sciences and Biotechnology,
    Tsinghua University, Beijing 100084, China
    b Multidisciplinary Research Center, Shantou University, Shantou 515063, China
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-28 发布日期:2007-06-28
  • 通讯作者: 陈晶瑜

Biosynthesis and characterization of polyhydroxyalkanoate copolyesters in Ralstonia
eutropha
PHB4 harboring a low-substrate-specificity PHA synthase PhaC2Ps from Pseudomonas
stutzeri
1317

CHEN Jingyua; ZHANG Leia; CHEN Jinchuna; CHEN Guoqianga,b   

  1. a MOE Laboratory of Protein Science, Department of Biological Sciences and Biotechnology,
    Tsinghua University, Beijing 100084, China
    b Multidisciplinary Research Center, Shantou University, Shantou 515063, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-28 Published:2007-06-28
  • Contact: CHEN Jingyu

摘要: A series of polyhydroxyalkanoate (PHA) copolymers consisting of short-chain-length (SCL)
and medium-chain-length (MCL) 3-hydroxyalkanoate (3HA) monomers were synthesized in the
recombinant Ralstonia eutropha PHB-4 harboring a low-substrate-specificity PHA synthase
PhaC2Ps from Pseudomonas stutzeri 1317. These polyesters, whose monomer compositions varied
widely in chain length, were purified and characterized by acetone fractionation, nuclear
magnetic resonance (NMR), gel-permeation chromatography (GPC), and differential scanning
calorimetry (DSC). This was the first time that the physical properties of PHA copolymers
polymerized by PhaC2Ps were characterized. The results indicated that the variation in MCL
3HA contents did not have an obvious influence on the molecular weights of these PHA
copolymers but was effective in changing their physical properties. The variation in the
thermal property of PHA copolymers with 3-hydroxyoctanoate (3HO) content was also inves-
tigated in this study.

关键词: polyhydroxybutyrate;PHA synthase;physical property;Pseudomonas stutzeri

Abstract: A series of polyhydroxyalkanoate (PHA) copolymers consisting of short-chain-length (SCL)
and medium-chain-length (MCL) 3-hydroxyalkanoate (3HA) monomers were synthesized in the
recombinant Ralstonia eutropha PHB-4 harboring a low-substrate-specificity PHA synthase
PhaC2Ps from Pseudomonas stutzeri 1317. These polyesters, whose monomer compositions varied
widely in chain length, were purified and characterized by acetone fractionation, nuclear
magnetic resonance (NMR), gel-permeation chromatography (GPC), and differential scanning
calorimetry (DSC). This was the first time that the physical properties of PHA copolymers
polymerized by PhaC2Ps were characterized. The results indicated that the variation in MCL
3HA contents did not have an obvious influence on the molecular weights of these PHA
copolymers but was effective in changing their physical properties. The variation in the
thermal property of PHA copolymers with 3-hydroxyoctanoate (3HO) content was also inves-
tigated in this study.

Key words: polyhydroxybutyrate, PHA synthase, physical property, Pseudomonas stutzeri