CIESC Journal

• BIOTECHNOLOGY & BIOENGINEERING • 上一篇    下一篇

氮源对利迪链菌素生产及相关次级代谢物分布的影响

李良智; 乔斌; 元英进   

  1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology,
    Tianjin University, Tianjin 30072, China
  • 收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2007-06-28 发布日期:2007-06-28
  • 通讯作者: 李良智

Nitrogen sources affect streptolydigin production and related secondary metabolites
distribution of Streptomyces lydicus AS 4.2501

LI Liangzhi; QIAO Bin; YUAN Yingjin   

  1. Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology,
    Tianjin University, Tianjin 30072, China
  • Received:1900-01-01 Revised:1900-01-01 Online:2007-06-28 Published:2007-06-28
  • Contact: LI Liangzhi

摘要: The effects of nitrogen sources on streptolydigin production and distribution of secondary
metabolites were investigated for flask cultured S. lydicus AS 4.2501. When peptone,
asparamide, and glutamic acid were examined as the nitrogen source, respectively, liquid
chromatography-mass spectrometry (LC-MS) and photodiode array (PDA) analyses revealed the
formation of two analogues of streptolydigin in the fermentation broth. When soybean meal
was used as the source of nitrogen, three analogues of streptolydigin were detected. The
use of ammonium sulfate as a source of nitrogen resulted in a lower pH value of the
fermentation system, thus inhibiting streptolydigin biosynthesis and changing the metabolic
profiling. Among the nitrogen sources that were made use of, glutamic acid was most
favorable to the formation of streptolydigin. Simultaneously, this study also showed that
the changing nitrogen sources resulted in altering the production and relative ratios of
streptolydigin and its analogues.

关键词: Streptomyces lydicus;nitrogen source;analogue;secondary metabolite;streptolydigin

Abstract: The effects of nitrogen sources on streptolydigin production and distribution of secondary
metabolites were investigated for flask cultured S. lydicus AS 4.2501. When peptone,
asparamide, and glutamic acid were examined as the nitrogen source, respectively, liquid
chromatography-mass spectrometry (LC-MS) and photodiode array (PDA) analyses revealed the
formation of two analogues of streptolydigin in the fermentation broth. When soybean meal
was used as the source of nitrogen, three analogues of streptolydigin were detected. The
use of ammonium sulfate as a source of nitrogen resulted in a lower pH value of the
fermentation system, thus inhibiting streptolydigin biosynthesis and changing the metabolic
profiling. Among the nitrogen sources that were made use of, glutamic acid was most
favorable to the formation of streptolydigin. Simultaneously, this study also showed that
the changing nitrogen sources resulted in altering the production and relative ratios of
streptolydigin and its analogues.

Key words: Streptomyces lydicus, nitrogen source, analogue, secondary metabolite, streptolydigin