补料分批培养耐乙酸大肠杆菌生产人表皮生长因子

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补料分批培养耐乙酸大肠杆菌生产人表皮生长因子

韩云¹; 李志敏¹; 杜鹏²; 甘人宝²; 叶勤¹

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
² Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sci-ences, Shanghai 200031, China

收稿日期:2006-10-16 修回日期:1900-01-01 出版日期:2007-10-28 发布日期:2007-10-28
通讯作者: 韩云

Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli

HAN Yun¹; LI Zhimin¹; DU Peng²; GAN Renbao²; YE Qin¹

¹ State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China
² Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sci-ences, Shanghai 200031, China

Received:2006-10-16 Revised:1900-01-01 Online:2007-10-28 Published:2007-10-28
Contact: HAN Yun

摘要/Abstract

摘要： An acetate-tolerant mutant of Escherichia coli DH5α, DA19, was used for secretory production of human epidermal growth factor (hEGF) whose expression was under the control of phoA promoter. The recombinant cells were cultured in a chemically defined medium, and glucose was added at different specific provision rates during the growth and expression phases. It was found that pH had a significant effect on the extracellular hEGF production. The extracellular hEGF concentration was 75.5mg•L－1, 5.2-fold of the level reached at pH 7.0, even though more acetate was produced. Nitrogen source was limited in the later growth phase. Supplementation of ammonium promoted the consumption of phosphate and reduced the time to exhaust phosphate, but the extracellular hEGF production was similar to that without supplementation of ammonium.

关键词: human epidermal growth factor;acetate-tolerance;Escherichia coli;pH

Abstract: An acetate-tolerant mutant of Escherichia coli DH5α, DA19, was used for secretory production of human epidermal growth factor (hEGF) whose expression was under the control of phoA promoter. The recombinant cells were cultured in a chemically defined medium, and glucose was added at different specific provision rates during the growth and expression phases. It was found that pH had a significant effect on the extracellular hEGF production. The extracellular hEGF concentration was 75.5mg•L－1, 5.2-fold of the level reached at pH 7.0, even though more acetate was produced. Nitrogen source was limited in the later growth phase. Supplementation of ammonium promoted the consumption of phosphate and reduced the time to exhaust phosphate, but the extracellular hEGF production was similar to that without supplementation of ammonium.

Key words: human epidermal growth factor, acetate-tolerance, Escherichia coli, pH

韩云, 李志敏, 杜鹏, 甘人宝, 叶勤. 补料分批培养耐乙酸大肠杆菌生产人表皮生长因子[J]. CIESC Journal.

HAN Yun, LI Zhimin, DU Peng, GAN Renbao, YE Qin. Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli[J]. .

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补料分批培养耐乙酸大肠杆菌生产人表皮生长因子

Production of human epidermal growth factor in fed-batch culture of acetate-tolerant Escherichia coli

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