蛋白质组学方法分析不同苯酚浓度下菌株Acinetobacter sp. EDP3的应激机理

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蛋白质组学方法分析不同苯酚浓度下菌株Acinetobacter sp. EDP3的应激机理

GENG Anli; LIM Ci Ji

School of Life Science and Chemical Technology, Ngee Ann Polytechnic, Singapore

收稿日期:2006-12-26 修回日期:1900-01-01 出版日期:2007-12-28 发布日期:2007-12-28
通讯作者: GENG Anli

Proteome analysis of the adaptation of a phenol-degrading bacterium Acinetobacter sp. EDP3 to the variation of phenol loadings

GENG Anli; LIM Ci Ji

School of Life Science and Chemical Technology, Ngee Ann Polytechnic, Singapore

Received:2006-12-26 Revised:1900-01-01 Online:2007-12-28 Published:2007-12-28
Contact: GENG Anli

摘要/Abstract

摘要： Strain EDP3 was isolated from an industrial-activated sludge. It belonged to the gamma group of Proteobacteria with an identity of 97.0% to Acinetobacter calcoaceticus according to the 16S rRNA gene sequences. It can tolerate up to 1000mg•L－1 phenol at room temperature with a much longer lag phase. This indicates that higher phenol concentration has induced some physiological and genotypic changes in the bacterium. The aim of this study is, therefore, to investigate these responses to phenol concentration variations in strain EDP3. Proteome analysis is conducted by means of a two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was conducted to obtain a deeper insight into the adaptive responses inside the bacterium. Comparative analysis of the proteome profiles of strain EDP3 grown in 400mg•L－1 and 1000mg•L－1 phenol allowed us to identify that among all the proteins up-regulated under the higher phenol concentration, oxidative stress proteins were dominant. The synthesis of a heat shock protein, 60000 chaperonin GroEL, was also amplified. In addition, the expression of one membrane protein, adenosine 5′-triphosphate (ATP)-binding cassette (ABC) type sugar transporter, was found up-regulated. The inhibition of adenosine 5′-triphosphate (ATP) and RNA/protein synthesis was also observed.

关键词: adaptation;phenol-degrading bacteria;Acinetobacter sp.;proteome

Abstract: Strain EDP3 was isolated from an industrial-activated sludge. It belonged to the gamma group of Proteobacteria with an identity of 97.0% to Acinetobacter calcoaceticus according to the 16S rRNA gene sequences. It can tolerate up to 1000mg•L－1 phenol at room temperature with a much longer lag phase. This indicates that higher phenol concentration has induced some physiological and genotypic changes in the bacterium. The aim of this study is, therefore, to investigate these responses to phenol concentration variations in strain EDP3. Proteome analysis is conducted by means of a two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and matrix-assisted laser desorption ionization time of flight mass spectrometry (MALDI-TOF-MS) was conducted to obtain a deeper insight into the adaptive responses inside the bacterium. Comparative analysis of the proteome profiles of strain EDP3 grown in 400mg•L－1 and 1000mg•L－1 phenol allowed us to identify that among all the proteins up-regulated under the higher phenol concentration, oxidative stress proteins were dominant. The synthesis of a heat shock protein, 60000 chaperonin GroEL, was also amplified. In addition, the expression of one membrane protein, adenosine 5′-triphosphate (ATP)-binding cassette (ABC) type sugar transporter, was found up-regulated. The inhibition of adenosine 5′-triphosphate (ATP) and RNA/protein synthesis was also observed.

Key words: adaptation, phenol-degrading bacteria, Acinetobacter sp., proteome

GENG Anli, LIM Ci Ji. 蛋白质组学方法分析不同苯酚浓度下菌株Acinetobacter sp. EDP3的应激机理[J]. CIESC Journal.

GENG Anli, LIM Ci Ji. Proteome analysis of the adaptation of a phenol-degrading bacterium Acinetobacter sp. EDP3 to the variation of phenol loadings[J]. .

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蛋白质组学方法分析不同苯酚浓度下菌株Acinetobacter sp. EDP3的应激机理

Proteome analysis of the adaptation of a phenol-degrading bacterium Acinetobacter sp. EDP3 to the variation of phenol loadings

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