碲化镉量子点与金纳米粒子用于DNA检测

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碲化镉量子点与金纳米粒子用于DNA检测

代昭¹; 张纪梅¹; 董全喜¹; 郭宁¹; 许世超¹; 孙波²; 步月华¹

¹ Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Material Science and Chemical Engi-neering, Tianjin Polytechnic University, Tianjin 300160, China
² Department of Material Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China

收稿日期:2007-01-08 修回日期:1900-01-01 出版日期:2007-12-28 发布日期:2007-12-28
通讯作者: 代昭

Adaption of Au nanoparticles and CdTe quantum dots in DNA detection

DAI Zhao¹; ZHANG Jimei¹; DONG Quanxi¹; GUO Ning¹; XU Shichao¹; SUN Bo²; BU Yuehua¹

¹ Tianjin Key Laboratory of Fiber Modification and Functional Fiber, School of Material Science and Chemical Engi-neering, Tianjin Polytechnic University, Tianjin 300160, China
² Department of Material Chemistry, College of Chemistry, Nankai University, Tianjin 300071, China

Received:2007-01-08 Revised:1900-01-01 Online:2007-12-28 Published:2007-12-28
Contact: DAI Zhao

摘要/Abstract

摘要： A DNA fluorescence probe system based on fluorescence resonance energy transfer (FRET) from CdTe quantum dot (QD) donors to Au nanoparticle (AuNP) acceptors is presented. CdTe QDs, 2.5nm in diameter, as energy donors, were prepared in water. Au nanoparticles, 16nm in diameter, as energy acceptors, were prepared from gold chloride by reduction. CdTe QDs were linked to 5′-NH2-DNA through 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC) as a linker, and the 3′-SH-DNA was self-assembled onto the surface of AuNPs. The hy-bridization of complementary double stranded DNA (dsDNA) bound to the QDs and AuNPs (CdTe-dsDNA-Au) determined the FRET distance of CdTe QDs and Au nanoparticles. Compared to the fluorescence of CdTe-DNA, the fluorescence of CdTe-DNA-Au conjugates decreased extremely, which indicated that the FRET occurred be-tween CdTe QDs and Au nanoparticles. The fluorescence change of this conjugate depended on the ratio of Au-DNA to CdTe-DNA. When the AuNPs-DNA to QD-DNA ratio was 10:1, the FRET efficiency reached a maxi-mum. The probe system would have a certain degree of fluorescence recovery when a complementary single stranded DNA was introduced into this system, which showed that the distance between CdTe QDs and Au nanoparticles was increased.

关键词: CdTe;quantum dots;Au nanoparticle;fluorescence resonance energy transfer;DNA

Abstract: A DNA fluorescence probe system based on fluorescence resonance energy transfer (FRET) from CdTe quantum dot (QD) donors to Au nanoparticle (AuNP) acceptors is presented. CdTe QDs, 2.5nm in diameter, as energy donors, were prepared in water. Au nanoparticles, 16nm in diameter, as energy acceptors, were prepared from gold chloride by reduction. CdTe QDs were linked to 5′-NH2-DNA through 1-ethyl-3-(dimethylaminopropyl)carbodiimide hydrochloride (EDC) as a linker, and the 3′-SH-DNA was self-assembled onto the surface of AuNPs. The hy-bridization of complementary double stranded DNA (dsDNA) bound to the QDs and AuNPs (CdTe-dsDNA-Au) determined the FRET distance of CdTe QDs and Au nanoparticles. Compared to the fluorescence of CdTe-DNA, the fluorescence of CdTe-DNA-Au conjugates decreased extremely, which indicated that the FRET occurred be-tween CdTe QDs and Au nanoparticles. The fluorescence change of this conjugate depended on the ratio of Au-DNA to CdTe-DNA. When the AuNPs-DNA to QD-DNA ratio was 10:1, the FRET efficiency reached a maxi-mum. The probe system would have a certain degree of fluorescence recovery when a complementary single stranded DNA was introduced into this system, which showed that the distance between CdTe QDs and Au nanoparticles was increased.

Key words: CdTe, quantum dots, Au nanoparticle, fluorescence resonance energy transfer, DNA

代昭, 张纪梅, 董全喜, 郭宁, 许世超, 孙波, 步月华. 碲化镉量子点与金纳米粒子用于DNA检测[J]. CIESC Journal.

DAI Zhao, ZHANG Jimei, DONG Quanxi, GUO Ning, XU Shichao, SUN Bo, BU Yuehua. Adaption of Au nanoparticles and CdTe quantum dots in DNA detection[J]. .

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碲化镉量子点与金纳米粒子用于DNA检测

Adaption of Au nanoparticles and CdTe quantum dots in DNA detection

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