CIESC Journal

• SEPARATION SCIENCE & ENGINEERING • 上一篇    下一篇

大孔吸附树脂精制鱼腥草黄酮类化合物

张颖; 李淑芬; 吴希文; 赵星   

  1. Key Laboratory for Green Chemical Technology of State Education Ministry, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • 收稿日期:2006-11-28 修回日期:1900-01-01 出版日期:2007-12-28 发布日期:2007-12-28
  • 通讯作者: 张颖

Macroporous resin adsorption for purification of flavonoids in Houttuynia cordata Thunb.

ZHANG Ying; LI Shufen; WU Xiwen; ZHAO Xing   

  1. Key Laboratory for Green Chemical Technology of State Education Ministry, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China
  • Received:2006-11-28 Revised:1900-01-01 Online:2007-12-28 Published:2007-12-28
  • Contact: ZHANG Ying

摘要: Flavonoids are one main kind of effective components in Houttuynia cordata Thunb., which display a wide range of pharmacological activity. In this study supercritical fluid extraction (SFE) with carbon dioxide was first used as preparation step to remove the volatile components, which are also active components, from Houttuynia cordata Thunb. Then ultrasound-assisted extraction was used to obtain the crude flavonoids and the macroporous resin adsorption technology was further employed to purify the flavonoids. Nine kinds of macroporous resins with different properties were tested through static adsorption, and one macroporous resin labeled as D101 was selected. The effect of several factors, such as the ratio of column height to diameter, initial concentration and pH, on both flavonoids yield and content were explored by dynamic adsorption to obtain reasonable conditions of adsorption and desorption. The experimental results show that the content of flavonoids can be above 60% with flavonoids recovery of 93.3 % under the optimum conditions of purification. HPLC analysis of the final flavonoids product shows it contains quercitrin, hyperin, rutin and quercetin.

关键词: flavonoids;Houttuynia cordata Thunb.;macroporous resin;adsorption;supercritical fluid extraction

Abstract: Flavonoids are one main kind of effective components in Houttuynia cordata Thunb., which display a wide range of pharmacological activity. In this study supercritical fluid extraction (SFE) with carbon dioxide was first used as preparation step to remove the volatile components, which are also active components, from Houttuynia cordata Thunb. Then ultrasound-assisted extraction was used to obtain the crude flavonoids and the macroporous resin adsorption technology was further employed to purify the flavonoids. Nine kinds of macroporous resins with different properties were tested through static adsorption, and one macroporous resin labeled as D101 was selected. The effect of several factors, such as the ratio of column height to diameter, initial concentration and pH, on both flavonoids yield and content were explored by dynamic adsorption to obtain reasonable conditions of adsorption and desorption. The experimental results show that the content of flavonoids can be above 60% with flavonoids recovery of 93.3 % under the optimum conditions of purification. HPLC analysis of the final flavonoids product shows it contains quercitrin, hyperin, rutin and quercetin.

Key words: flavonoids, Houttuynia cordata Thunb., macroporous resin, adsorption, supercritical fluid extraction