Abstract:

The optimal conditions of cetyl trimethyl ammonium bromide (CTAB) reverse micellar system, and the most suitable reaction conditions of synthesis of ethyl oleate by Lipex lipase were determined. The influences of organic solvent, ratio of substrates, pH value, water content W₀, concentration of oleic acid and reaction time were investigated. As a result, a more stable reverse micellar system was obtained, which led to a better activity of Lipex lipase. The parameters in this condition were optimized as follows: reaction temperature 25 ℃, buffer pH value 7. 0, W₀ 10, ratio of acid to ethanol 1∶4, oleic acid concentration 0. 01 mol·L^-1, agitation speed 150 r·min^-1 and reaction time 36 h, and the yield of ethyl oleate was 79%. The kinetic model of the reverse micellar system influenced by double substrates was established. The simulated result agreed well with the experimental result.