Abstract:

Sugar was selected for protecting Candida antarctic lipase B entrapped in sol-gel glass matrix. The activities of immobilized CALB influenced by sucrose, glucose, trehalose, xylose and maltose were investigated. The result showed that trehalose,glucose and xylose were the best protector for lipase CALB. By means of infrared spectrometry and differential scanning calorimeter，the protection mechanism of immobilized CALB by xylose was studied. The results showed that xylose bonded with lipase provided significant improvement in the stability of entrapped CALB.