Abstract:

The microstructure，swelling ratio and protein concentration and separation behavior of dextran bulk hydrogels with microstructure（BHMs）were studied with scanning electron microscopy（SEM），visible ultra-violet spectrophotometry（UV）and high performance liquid chromatography（HPLC）.The experimental results indicated that the bovine serum albumin concentration and separation efficiency and swelling ratio increased with increasing microgels content or decreasing temperature. Comparing with the cross-linked dextran hydrogels，dextran BHMs realized baseline separation for multi-component proteins including bovine serum albumin，lysozyme and ovalbumin; additionally，the separation time was reduced from 24 h to 9 h. The excellent concentration and separation performance of dextran BHMs were ascribed to its loose and porous microstructure.