Abstract:

The theory of metabolic engineering was applied to a fed batch culture of animal cells. Through the construction of metabolic network model, the time distribution of mass and energy metabolic fluxes was studied for hybridoma cells from dynamic to pseudo-steady state. The distribution partition of mass metabolic flux of high-level glucose between lactate production pathway and tricarboxylic acid (TCA) cycle was 90% and 10%, with the ratio of ATP production 20% and 60%, respectively. When glucose concentration decreased below the growth-limiting extent, the percentage of glucose entering into TCA cycle was gradually increased to 100%, the oxidative extent of glucose was improved from 0. 6 mmol·mmol^-1 to 4. 52 mmol·mmol^-1. It suggested that glucose metabolism was drifted from anaerobic lactate production pathway to aerobic TCA cycle.