Abstract: A novel method was developed to fabricate engineered tissue implants through re-aggregation of micro-tissues. Furthermore, experimental research and CFD modeling were carried out to validate this idea.The method included the following steps:(1)Culturing the cells on the macro-pore micro-carriers to form micro-tissues;(2)Concentrating the micro-tissues in a perfusion bioreactor having the geometry desired for implantation;(3)Further culturing the micro-tissues to cause the formation of a consolidated large construct.The experimental results showed that this method could enhance cell number density, improve cell distribution and extra-cellular matrix synthesis.The CFD modeling also indicated that the fluid dynamics environment and mass transport condition improved significantly.This method is beneficial to cells in micro-carriers and will reduce limitations of nutrients and oxygen.