Abstract: The removal of hydrogen sulfide was carried out under optimized conditions，and the absorbent was bacterial cultures of Thiobacillus ferrooxidans or acid solution of Fe2（SO4）3. In order to provide valuable reference for technical design of sulfur recovery in natural gas industry，the properties of by-product sulfur from the bio-oxidation of hydrogen sulfide were investigated. The results indicated that the efficiency of removing hydrogen sulfide by bacterial cultures was over 90% after reacting for 45 min，and higher than that by acid solution of Fe2（SO4）3. The by-product sulfur was insoluble in water，partially soluble in ethanol，and completely soluble in carbon disulfide and carbon tetrachloride. It had a specific density of 1. 90 g·cm-3 and melting point of 121℃. The shape of the particles was irregular globular, in agglomeration. The particle mean size was 5. 09 μm after dispersion. The by-product sulfur was hydrophilic，and better than hydrophobic sublimed sulfur, or the by-product sulfur from hydrogen sulfide removal by acid solution of Fe2 (SO4)3.The settling velocity of sulfur particles was 0. 125×10-2m·s-1 as shown in an intermittent sedimentation test.