关键词:

己烯雌酚, 氯乙酸钠, 牛血清蛋白, 混合酸酐法, 完全抗原, 抗体

Abstract:

Diethylstilbestrol monocarboxymethoxyflurane (DES-MCME) was synthesized by first retouching a phenolic hydroxyl group of DES molecule and using diethylstilbestrol;sodium monochloroacetate and bovine serum albumin as key raw materials.The conjugations of DES-MCME with proteins as a complete antigen were prepared by the mixed acid anhydride method and the conjugations were analyzed by ultraviolet scanning.According to the immune protocol;New Zealand white rabbits were immunized.The results of experimentation showed that isomerism was present in cis-and trans-forms of DES and its derivates;which was effected obviously by polar solvents.High resolution mass spectroscopy revealed that the molecular mass of DES-MCME was 325.The optimal conjugation ratios of immunogen and coating antigen were 13 and 10.The dilution rate of antiserum could reach 102400;through an indirect enzyme-linked immunosorbent assay (ELISA).The preparation method of DES-MCME was developed.The effectiveness of the artificial antigen of DES was established by high resolution mass spectroscopy and ELISA methods.This could provide the basis for the DES ELISA kit.

Key words:

己烯雌酚, 氯乙酸钠, 牛血清蛋白, 混合酸酐法, 完全抗原, 抗体