Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet

CIESC Journal ›› 2009, Vol. 17 ›› Issue (3): 373-380.

Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet

周冉, 李淑芬, 张大成

Key Laboratory for Green Chemical Technologies of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

收稿日期:2008-09-11 修回日期:2009-02-17 出版日期:2009-06-28 发布日期:2009-06-28
通讯作者: LI Shufen,E-mail:shfli@tju.edu.cn
基金资助:
Supported by the Natural Science Foundation of Tianjin (06YFJMJC10500)

Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet

ZHOU Ran, LI Shufen, ZHANG Dacheng

Key Laboratory for Green Chemical Technologies of the Ministry of Education, School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China

Received:2008-09-11 Revised:2009-02-17 Online:2009-06-28 Published:2009-06-28
Supported by:
Supported by the Natural Science Foundation of Tianjin (06YFJMJC10500)

摘要/Abstract

摘要： Supercritical CO₂ (SC-CO₂) extraction technology and ultrasonic technology were used to extract two active sex hormones, estradiol and progesterone, and insulin-like growth factor-1 (IGF-1) from antler velvet. The effects of SC-CO₂ extraction condition on the extraction yield and content of sex hormones, the ultrasonic extraction condition on the content of IGF-1 and the SC-CO₂ extraction condition on the activity remaining of IGF-1 were studied. The optimal conditions were obtained. The experimental results showed that, in presence of 75% ethanol as the co-solvent, the mean yield and content of estradiol and progesterone were 87.67 pg·g^-1 and 1224.10 pg·g^-1, 12.38 ng·g^-1 and 354.06 ng·g^-1, respectively, with extraction pressure of 30 MPa, temperature of 35℃, extraction time of 30 min and CO₂ consumption of 15 L·g^-1 at the flow rate of 2.0 L·min^-1. The highest content of IGF-1 was 7425.75 ng·g^-1 antler velvet residue, when the pH10 ammonia-ammonium chloride buffer solution was used as the solvent, the ratio of solvent to sample was 20/1 (volume/mass), the extraction temperature was 0-35℃, and the extraction time was 4×15 min. Under these conditions, 93.68% activity remaining of IGF-1 in the residue was obtained, while little IGF-1 activity exists in traditional residue. The experimental results indicate that the technology of SC-CO₂ with co-solvent is of advantage for getting high content sexual hormones and keeping high activity of IGF-1 in the residue, which can not be achieved by traditional extraction methods.

关键词: antler velvet, supercritical extraction, estradiol, progesterone, insulin-like growth factor-1

Abstract: Supercritical CO₂ (SC-CO₂) extraction technology and ultrasonic technology were used to extract two active sex hormones, estradiol and progesterone, and insulin-like growth factor-1 (IGF-1) from antler velvet. The effects of SC-CO₂ extraction condition on the extraction yield and content of sex hormones, the ultrasonic extraction condition on the content of IGF-1 and the SC-CO₂ extraction condition on the activity remaining of IGF-1 were studied. The optimal conditions were obtained. The experimental results showed that, in presence of 75% ethanol as the co-solvent, the mean yield and content of estradiol and progesterone were 87.67 pg·g^-1 and 1224.10 pg·g^-1, 12.38 ng·g^-1 and 354.06 ng·g^-1, respectively, with extraction pressure of 30 MPa, temperature of 35℃, extraction time of 30 min and CO₂ consumption of 15 L·g^-1 at the flow rate of 2.0 L·min^-1. The highest content of IGF-1 was 7425.75 ng·g^-1 antler velvet residue, when the pH10 ammonia-ammonium chloride buffer solution was used as the solvent, the ratio of solvent to sample was 20/1 (volume/mass), the extraction temperature was 0-35℃, and the extraction time was 4×15 min. Under these conditions, 93.68% activity remaining of IGF-1 in the residue was obtained, while little IGF-1 activity exists in traditional residue. The experimental results indicate that the technology of SC-CO₂ with co-solvent is of advantage for getting high content sexual hormones and keeping high activity of IGF-1 in the residue, which can not be achieved by traditional extraction methods.

Key words: antler velvet, supercritical extraction, estradiol, progesterone, insulin-like growth factor-1

周冉, 李淑芬, 张大成. Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet[J]. CIESC Journal, 2009, 17(3): 373-380.

ZHOU Ran, LI Shufen, ZHANG Dacheng. Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet[J]. , 2009, 17(3): 373-380.

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Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet

Combination of Supercritical Fluid Extraction with Ultrasonic Extraction for Obtaining Sex Hormones and IGF-1 from Antler Velvet

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