Abstract: Straight-run gasoline separation was studied with a novel column structure, the dividing wall column. This process was simulated with a commercial software HYSYS, and then the optimized design parameters were obtained. Based on these parameters, operation conditions such as the reflux ratio and the liquid split ratio were investigated with a pilot plant. Experimental results revealed that a higher reflux ratio is beneficial to the product separation, while the energy consumption is relatively higher. Also, the liquid split ratio has a wider range for selection, which is an important factor for the product quality.