关键词:

重组大肠杆菌, 高密度发酵, 大规模培养

Abstract:

The scale-up of fermentation process plays an important role in the industrialization of lab results.As the increase of fermentation scale, the microenvironment of microorganism would be different, which would result in the change of microorganism metabolism and affect the results of scale-up.In order to accomplish the industrialization of HT-1 production, high-expression of HT-1 fusion protein was carried out with fed-batch culture method of E.coli in a 200 L fermentor.The problem of the decrease in HT^-1 fusion protein expression level caused by secondary seed culture process was solved by the methods of advancing initial induction and increasing inoculation volume.As a result, the cell density, the expression level, the cell yield as well as the volume yield of HT-1 fusion protein were achieved to 55.1 g·L^-1, 27.4%, 0.056 g·(g cell)^-1 and 3.103 g·L^-1, respectively.Compared with the results before optimization, these indexes were increased by 9.8%, 23.4%, 14.3% and 25.2%, respectively.These results provide an important reference for the optimization and scale-up of HT-1 production.

Key words:

重组大肠杆菌, 高密度发酵, 大规模培养