Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05

CIESC Journal ›› 2010, Vol. 18 ›› Issue (1): 108-112.

Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05

王俊峰^1,2, 吴鹏飞¹, 陈建¹, 闫海¹

1. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China;
2. Department of Environmental Engineering, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China

收稿日期:2009-02-10 修回日期:2009-06-23 出版日期:2010-02-28 发布日期:2010-12-30
通讯作者: YAN Hai, E-mail: haiyan@sas.ustb.edu.cn
作者简介:
基金资助:
Supported by the State Key Development Program for Basic Research of China(2008CB418105);the National Natural Science Foundation of China(203777008,20621703);the State Key Joint Laboratory of Environment Simulation and Pollution Control(09K08ESPCT);the Educational Committee of Beijing

Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05

WANG Junfeng^1,2, WU Pengfei¹, CHEN Jian¹, YAN Hai¹

1. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China;
2. Department of Environmental Engineering, School of Resource and Environmental Engineering, Jiangxi University of Science and Technology, Ganzhou 341000, China

Received:2009-02-10 Revised:2009-06-23 Online:2010-02-28 Published:2010-12-30
Supported by:
Supported by the State Key Development Program for Basic Research of China(2008CB418105);the National Natural Science Foundation of China(203777008,20621703);the State Key Joint Laboratory of Environment Simulation and Pollution Control(09K08ESPCT);the Educational Committee of Beijing

摘要/Abstract

摘要： A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3mg·L^-1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract(CE)of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3mg·L^-1 was completely biodegraded within 10h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.

关键词: microcystin-RR, Sphingopyxis sp.USTB-05, cell-free extract, biodegradation

Abstract: A promising bacterial strain for the biodegradation of Microcystins(MCs)was isolated from Dianchi lake in China and identified as Sphingopyxis sp.USTB-05 by the analysis of 16s rDNA.Initial MC-RR of 42.3mg·L^-1 was completely degraded by USTB-05 within 36 h,which was a relatively high biodegradation rate of MC-RR.With the cell-free extract(CE)of Sphingopyxis sp.USTB-05,MC-RR was biodegraded at a more rapid biodegradation rate compared with its strain,so that initial MC-RR of 42.3mg·L^-1 was completely biodegraded within 10h.During the bio-reaction of MC-RR catalyzed by CE,two intermediate metabolites and a dead-end product of MC-RR were observed on HPLC profiles and all of them had similar scanning profiles in the wavelength from 200 to 300 nm,indicating that the group of Adda in all products of MC-RR remained intact.

Key words: microcystin-RR, Sphingopyxis sp.USTB-05, cell-free extract, biodegradation

王俊峰, 吴鹏飞, 陈建, 闫海. Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05[J]. CIESC Journal, 2010, 18(1): 108-112.

WANG Junfeng, WU Pengfei, CHEN Jian, YAN Hai. Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05[J]. , 2010, 18(1): 108-112.

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Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05

Biodegradation of Microcystin-RR by a New Isolated Sphingopyxis sp. USTB-05

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