Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology

CIESC Journal ›› 2010, Vol. 18 ›› Issue (1): 137-142.

Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology

张驰^1,2,3, 范代娣^2,3,4, 尚龙安⁵, 马晓轩^2,3,4, 骆艳娥^2,3,4, 薛文娇^2,3,4, 高鹏飞^2,3,4

1. School of Life Science, Northwest University, Xi'an 710069, China;
2. Shaanxi R&D Center of Biomaterial and Fermentation, Xi'an 710069, China ;
3. Shaanxi Key Laboratory of Degradable Biomedical Materials, Xi'an 710069, China;
4. School of Chemical Engineering, Northwest University, Xi'an 710069, China;
5. College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China

收稿日期:2009-03-28 修回日期:2009-08-09 出版日期:2010-02-28 发布日期:2010-12-30
通讯作者: FAN Daidi, E-mail: fandaidi@nwu.edu.cn
作者简介:
基金资助:
Supported by the National Natural Science Foundation of China(20776119);the National High Technology Research and Development Program of China(2007AA03Z456A);the Special Research Program of the Education Department of Shaanxi Province(07JK417)

Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology

ZHANG Chi^1,2,3, FAN Daidi^2,3,4, SHANG Long'an⁵, MA Xiaoxuan^2,3,4, LUO Yan'e^2,3,4, XUE Wenjiao^2,3,4, GAO Pengfei^2,3,4

1. School of Life Science, Northwest University, Xi'an 710069, China;
2. Shaanxi R&D Center of Biomaterial and Fermentation, Xi'an 710069, China ;
3. Shaanxi Key Laboratory of Degradable Biomedical Materials, Xi'an 710069, China;
4. School of Chemical Engineering, Northwest University, Xi'an 710069, China;
5. College of Biological and Chemical Engineering, Ningbo Institute of Technology, Zhejiang University, Ningbo 315100, China

Received:2009-03-28 Revised:2009-08-09 Online:2010-02-28 Published:2010-12-30
Supported by:
Supported by the National Natural Science Foundation of China(20776119);the National High Technology Research and Development Program of China(2007AA03Z456A);the Special Research Program of the Education Department of Shaanxi Province(07JK417)

摘要/Abstract

摘要： In order to improve the production of human-like collagen Ⅲ(HLC Ⅲ)by fed-batch culture of recombinant Escherichia coli BL21,the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters.Three variables(induction time,inoculum age and pH),which have significant effects on HLC Ⅲ production,were selected from eight variables by Plackett-Burman design.With the regression coefficient analysis in the Box-Behnken design,a relationship between HLC Ⅲ production and three significant factors was obtained,and the optimum levels of the three variables were as follows:induction time 3.2h,inoculum age 12.6 h and pH 6.7.The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant.An average 9.68 g·L^-1HLC Ⅲ production was attained in the validation experiment under optimized condition,which was 80%higher than the yield of 5.36 g·L^-1 before optimization.

关键词: ermentation process parameters, human-like collagen Ⅲ, optimization, response surface methodology

Abstract: In order to improve the production of human-like collagen Ⅲ(HLC Ⅲ)by fed-batch culture of recombinant Escherichia coli BL21,the Plackett-Burman and Box-Behnken design were applied to optimize the fermentation process parameters.Three variables(induction time,inoculum age and pH),which have significant effects on HLC Ⅲ production,were selected from eight variables by Plackett-Burman design.With the regression coefficient analysis in the Box-Behnken design,a relationship between HLC Ⅲ production and three significant factors was obtained,and the optimum levels of the three variables were as follows:induction time 3.2h,inoculum age 12.6 h and pH 6.7.The 3D response surface plots and 2D contour plots created by the Box-Behnken design showed that the interaction between induction time and pH and that between innoculum age and pH were significant.An average 9.68 g·L^-1HLC Ⅲ production was attained in the validation experiment under optimized condition,which was 80%higher than the yield of 5.36 g·L^-1before optimization.

Key words: ermentation process parameters, human-like collagen Ⅲ, optimization, response surface methodology

张驰, 范代娣, 尚龙安, 马晓轩, 骆艳娥, 薛文娇, 高鹏飞. Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology[J]. CIESC Journal, 2010, 18(1): 137-142.

ZHANG Chi, FAN Daidi, SHANG Long'an, MA Xiaoxuan, LUO Yan'e, XUE Wenjiao, GAO Pengfei. Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology[J]. , 2010, 18(1): 137-142.

参考文献

1 Jenkins, C.L., Raines, R.T., “Insights on the conformational stability of collagen”, Nat. Product Rep., 19, 49-59 (2002).
2 Hua, X.F., Fan, D.D., Luo, Y.E., Zhang, X., Shi, H.J., Ma, X.X., Shang, L.A., “Kinetics of high cell density fed-batch culture of recombinant Escherichia coli producing human-like collagen”, Chin. J. Chem. Eng., 14, 242-247 (2006).
Schlosser, S., Groger, G., Seliger, H., “Cloning and expression of collagen-derived protein in E. coli and baculovirus system”, Nucleosides Nucleotides, 17, 1515-1522 (1998).
4 Cappello, J., Ferrari, F.A., “High molecular weight collagen-like protein polymers”, U.S. Pat., 5773249 (1998).
5 Ruggiero, F., Exposito, J.Y., Bournat, P., Gruber, V., Perret, S., Comte, J., Olagnier, B., Garrone, R., Theisen, M., “Triple helix assembly and processing of human collagen produced in transgenic tobacco plants”, FEBS Lett., 469, 132-136 (2000).
6 Ruggiero, F., Chanut, H., Fichard, A., “Production of recombinant collagen for biomedical devices”, Pharm. Technol., 24, 26-38 (2000).
7 Fan, D.D., Luo, Y.E., Mi, Y., Ma, X.X., Shang, L.A., “Characteristics of fed-batch cultures of recombinant Escherichia coli containing human-like collagen cDNA at different specific growth rates”, Biotechnol. Lett., 27, 865-870 (2005).
8 Huang, C.J., Chen, X.Y., “High-level expression and characterization of two chitinases, ChiCH and ChiCW, of Bacillus cereus 28-9 in Escherichia coli”, Biochem. Biophys. Res. Commun., 327, 8-17 (2005).
9 Neubauer, A., Neubauer, P., Myllyharju, J., “High-level production of human collagen prolyl-4-hydroxylase in Escherichia coli”, Matrix Biol., 24, 59-68 (2005).
10 Gao, H.J., Wu, Q., Chen, G.Q., “Enhanced production of D-(—)-3-hydroxybutyric acid by recombinant Escherichia coli”, FEMS Microbiol. Lett., 213, 59-65 (2002).
11 Lu, Y.H., Deng, X., Cheng, Z.J., Li, Q.B., Liu, G., “Enhanced production of hybrid extracellular β-glucanase by recombinant Escherichia coli using experimental design method”, Chin. J. Chem. Eng., 15, 172-177 (2007).
12 Xu, Q.Q., Lü, L.X., Chen, S.Y., Zheng, J.J., Zheng, G.L., Li, Y.Q., “Isolation of Cordyceps ophioglossoides L2 from fruit body and optimization of fermentation conditions for its mycelial growth”, Chin. J. Chem. Eng., 17, 278-285 (2009).
13 Reddy, L.V.A., Wee, Y.J., Yun, J.S., Ryu, H.W., “Optimization of alkaline protease production by batch culture of Bacillus sp. RKY3 through Plackett-Burman and response surface methodological approaches”, Bioresour. Technol., 99, 2242-2249 (2008).
14 Song, X.J., Zhang, X.C., Kuang, C.H., Zhu, L.Y., Guo, N., “Optimization of fermentation parameters for the biomass and DHA production of Schizochytrium limacinum OUC88 using response surface methodology”, Proc. Biochem., 42, 1391-1397 (2007).
15 Francis, F., Sabu, A., Nampoothiri, K.M., Ramachandran, S., Ghosh, S., Szakacs, G., Pandey, A.,“Use of response surface methodology for optimizing process parameters for the production of α-amylase by Aspergillus oryzae”, Biochem. Eng. J., 15, 107-115 (2003).
16 Shiloach, J., Fass, R., “Growing E. coli to high cell density—A historical perspective on method development”, Biotechnol. Adv., 23, 345-357 (2005).
17 Yin, M.W., Nan, Y.M., Wang, X.M., “Improvement of the spectrophotometric method for the determination of hydroxyproline”, J. Henan Med. Univ., 29, 74-77(1994). (in Chinese)
18 Purama, R.K., Goyal, A., “Screening and optimization of nutritional factors for higher dextransucrase production by leuconostoc mesenteroides NRRL B-40 using statistical approach”, Bioresour. Technol., 99, 7108-7114 (2008).
19 Haider, M.A., Pakshirajan, K., “Screening and optimization of media constituents for enhancing lipolytic activity by soil microorganism using statistically designed experiments”, Appl. Biochem. Biotechnol., 141, 377-390 (2007).
20 Stanbury, P.F., Hall, S., Whitaker, A., Principles of Fermentation Technology, Butterworth-Heinemann, London (1999).
21 McNeil, B., Harvey, L., Practical Fermentation Technology, Wiley, New York (2006).
22 Chu, J., Li, Y.R., Modern Biotechnology, East China University of Science and Technology Press, Shanghai (2006).

[1]	费正顺, 胡斌, 叶鲁彬, 梁军. 基于ARX-NNPLS模型的优化策略及在聚合物牌号切换过程中的应用[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 971-979.
[2]	罗剑飞, 林炜铁, 蔡小龙, 李敬源. 基于神经网络与遗传算法结合的硝化细菌发酵培养基优化[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 950-957.
[3]	杨婵媛, 朱晓丽, 范代娣, 米钰, 骆艳娥, 惠俊峰, 苏然. 长双歧杆菌BIOMA 5920的冻干保护剂配方的优化[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 930-936.
[4]	杨克迪, 谭芳香, 王凡, 龙云飞, 文衍宣. 响应面法优化碳热还原合成LiFePO₄/C的工艺参数[J]. Chinese Journal of Chemical Engineering, 2012, 20(4): 793-802.
[5]	吴新杰, 崔春阳, 胡晟, 李志宏, 吴成东. 基于粒子群优化算法和非线性盲源信号分离测量两相流速度[J]. CIESC Journal, 2012, 20(2): 346-351.
[6]	郑广俭, 崔学民, 张伟鹏, 童张法, 李峰. 具有固定硅铝比的Al₂O₃-2SiO₂纳米颗粒制备：过程优化与粉体转化[J]. CIESC Journal, 2012, 20(2): 312-318.
[7]	许勤勤, 刘振华, 孙一晟, 丁忠杰, 吕龙贤, 李永泉. 大团囊虫草菌胞内多糖发酵优化和抗氧化活性研究[J]. CIESC Journal, 2012, 20(2): 294-301.
[8]	焦云强, 苏宏业, 廖祖维, 侯卫锋. Modeling and Multi-objective Optimization of Refinery Hydrogen Network[J]. CIESC Journal, 2011, 19(6): 990-998.
[9]	胡纯铿, 秦晴, 高培培. Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation[J]. CIESC Journal, 2011, 19(6): 1017-1022.
[10]	Andreas Harwardt, Korbinian Kraemer, Bettina Rüngeler, Wolfgang Marquardt. Conceptual Design of a Butyl-levulinate Reactive Distillation Process by Incremental Refinement[J]. CIESC Journal, 2011, 19(3): 371-379.
[11]	Ignacio E. Grossmann, Mariano Martín. Energy and Water Optimization in Biofuel Plants[J]. CIESC Journal, 2010, 18(6): 914-922.
[12]	摆亮, 江永亨, 黄德先, 刘先广. A Novel Scheduling Strategy for Crude Oil Blending[J]. CIESC Journal, 2010, 18(5): 777-786.
[13]	王建林, 薛尧予, 于涛, 赵利强. Run-to-run Optimization for Fed-batch Fermentation Process with Swarm Energy Conservation Particle Swarm Optimization Algorithm[J]. CIESC Journal, 2010, 18(5): 787-794.
[14]	李政, 邓利, 鲁吉珂, 郭小雷, 杨自信, 谭天伟. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. CIESC Journal, 2010, 18(5): 870-875.
[15]	郭佳庆, 骆艳娥, 范代娣, 高鹏飞, 马晓轩, 朱晨辉. Analysis of Metabolic Products by Response Surface Methodology for Production of Human-like Collagen II[J]. CIESC Journal, 2010, 18(5): 830-836.

Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology

Optimization of Fermentation Process for Human-like Collagen Production of Recombinant Escherichia coli Using Response Surface Methodology

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价