Preparation of Supermacroporous Composite Cryogel Embedded with SiO2 Nanoparticles

CIESC Journal ›› 2010, Vol. 18 ›› Issue (4): 667-671.

Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

许潘萍¹, 姚雨辰², 沈绍传¹, 贠军贤¹, 姚克俭¹

1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;
2. School of Chemical Engineering, University of New South Wales, Sydney NSW 2052, Australia

收稿日期:2010-01-13 修回日期:2010-04-23 出版日期:2010-08-28 发布日期:2010-08-28
通讯作者: YAO Kejian, E-mail:yaokj@zjut.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China (20876145);the Natural Science Foundation of Zhejiang Province (Y4080329)

Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

XU Panping¹, YAO Yuchen², SHEN Shaochuan¹, YUN Junxian¹, YAO Kejian¹

1. State Key Laboratory Breeding Base of Green Chemistry Synthesis Technology, College of Chemical Engineering and Materials Science, Zhejiang University of Technology, Hangzhou 310032, China;
2. School of Chemical Engineering, University of New South Wales, Sydney NSW 2052, Australia

Received:2010-01-13 Revised:2010-04-23 Online:2010-08-28 Published:2010-08-28
Supported by:
Supported by the National Natural Science Foundation of China (20876145);the Natural Science Foundation of Zhejiang Province (Y4080329)

摘要/Abstract

摘要： Supermacroporous composite cryogels embedded with SiO₂ nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide (AAm) and N,N-methylene-bis-acrylamide (MBAAm) containing SiO₂ nanoparticles (mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08) under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate (HETP) of the cryogel beds at different liquid flow rates was determined by residence time distribution (RTD) using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO₂ nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i. e.,15 cm·min^-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.

关键词: composite cryogel, SiO₂ nanoparticle, protein chromatography

Abstract: Supermacroporous composite cryogels embedded with SiO₂ nanoparticles were prepared by radical cryogenic copolymerization of the reactive monomer mixture of acrylamide (AAm) and N,N-methylene-bis-acrylamide (MBAAm) containing SiO₂ nanoparticles (mass ratios of nanoparticles to the monomer AAm from 0.01 to 0.08) under the freezing-temperature variation condition in glass columns.The properties of these composite cryogels were measured.The height equivalent to theoretical plate (HETP) of the cryogel beds at different liquid flow rates was determined by residence time distribution (RTD) using tracer pulse-response method.The composite cryogel matrix embedded with the mass fraction of SiO₂ nanoparticles of 0.02 presented the best properties and was employed in the following graft polymerization.Chromatographic process of lysozyme in the composite cryogel grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid (AMPSA) was carried out to evaluate the protein breakthrough and elution characteristics.The chromatography can be carried out at relatively high superficial velocity,i. e.,15 cm·min^-1,indicating the satisfactory mechanical strength due to the embedded nanoparticles.

Key words: composite cryogel, SiO₂ nanoparticle, protein chromatography

许潘萍, 姚雨辰, 沈绍传, 贠军贤, 姚克俭. Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles[J]. CIESC Journal, 2010, 18(4): 667-671.

XU Panping, YAO Yuchen, SHEN Shaochuan, YUN Junxian, YAO Kejian. Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles[J]. , 2010, 18(4): 667-671.

参考文献

1 Lozinsky,V.I.,Galaev,I. Y.,Plieva,F. M.,Savina,I. N.,Jungvid,H., Mattiasson,B.,“Polymeric cryogels as promising materials of biotechnological interest”,Trends in Biotechnology,21,445-451 (2003). 2 Plieva,F. M.,Galaev,I.Y.,Mattiasson,B.,“Macroporous gels prepared at subzero temperatures as novel materials for chromatography of particulate-containing fluids and cell culture applications”,J.Sep. Sci.,30,1657-1671(2007). 3 Plieva,F.M.,Mattiasson,B.,“Macroporous gel particles as novel sorbent materials:rational design”,Ind.Eng.Chem.Res.,47, 4131-4141(2008).4 He,X. J.,Yao,K. J.,Shen,S. C.,Yun,J. X.,“Freezing characteristics of acrylamide-based aqueous solution used for the preparation of supermacroporous cryogels via cryo-copolymerization”,Chem.Eng. Sci.,62,1334-1342(2007). 5 Yao,K. J.,Shen,S. C.,Yun,J. X.,Wang,L.H.,He,X. J.,Yu,X. M., “Preparation of polyacrylamide-based supermacroporous monolithic cryogel beds under freezing-temperature variation conditions”, Chem.Eng.Sci.,61,6701-6708(2006). 6 Yao,K.J.,Yun,J. X.,Shen,S.C.,Wang,L. H.,He,X. J.,Yu,X. M., “Characterization of a novel continuous supermacroporous monolithic cryogel embedded with nanoparticles for protein chromatography”,J.Chromatogr.A,1109,103-110 (2006). 7 Yao,K.J.,Yun,J.X.,Shen,S.C.,Chen,F.,“In-situ graft-polymerization preparation of cation-exchange supermacroporous cryogel with sulfo groups in glass columns”,J.Chromatogr.A,63,246-251 (2007). 8 Yao,K.J.,Yun,J.X.,Shen,S. C.,Wang,L. H.,Chen,F.,Yu,X. M., “Protein adsorption in supermacroporous cryogels with embedded nanoparticles”,Biochem.Eng.J.,36,139-146 (2007). 9 Chen,F.,Yao,K. J.,Shen,S. C.,Yun,J.X.,“Influence of grafting conditions on the properties of polyacrylamide-based cation-exchange cryogels grafted with 2-acrylamido-2-methyl-1-propanesulfonic acid”, Chem.Eng.Sci.,63,71-77 (2008). 10 Chen,Y.,Shen,S. C.,Yun,J.X.,Wang,L.H.,Yao,K. J.,Yao,S. J., “Isolation of ATP from a yeast fermentation broth using a cryogel column at high flow velocities”,J. Sep.Sci.,31,3879-3883(2008). 11 Wang,L.H.,Shen,S.C.,He,X.J.,Yun,J.X.,Yao,K.J.,Yao,S.J., “Adsorption and elution behaviors of bovine serum albumin in metal-chelated affinity cryogel beds”,Biochem.Eng.J.,42,237-242 (2008). 12 Jungbauer,A.,Hahn,R.,“Monoliths for fast bioseparation and bioconversion and their applications in biotechnology”,J.Sep.Sci.,27, 767-778 (2004). 13 Podgornik,A.,Jan?ar,J.,Merhar,M.,Kozamernik,S.,Glover,D., ?u?ek,K.,Barut,M.,?trancar,A.,“Large-scale methacrylate monolithic columns:design and properties”,J.Biochem.Biophys.Methods,60,179-189(2004). 14 Zaitsev,V.S.,Filimonov,D.S.,Presnyakov,I.A.,Gambino,R.J.,Chu, B.,“Physical and chemical properties of magnetite and magnetite-polymer nanoparticles and their colloidal dispersions”,J.Colloid Interf.Sci.,212,49-57(1999). 15 Haraguchi,K.,Farnworth,R.,Ohbayashi,A.,Takehisa,T.,“Compositional effects on mechanical properties of nanocomposite hydrogels composed of poly(N,N-dimethylacrylamide)and clay”, Macromolecules,36,5732-5741(2003). 16 Hu,Y.,Chen,J.F.,Zhang,H.T.,Li,T.W.,Xue,X.,“Using silicon dioxide nanosphere gaps to confine growth of single-crystal nickel sulfide nanowires in polyacrylamide gel”,Scripta Materialia,55, 131-134(2006). 17 Yu,S. L.,Zuo,X. T.,Bao,R. L.,Xu,X.,Wang,J.,Xu,J.,“Effect of SiO₂ nanoparticle addition on the characteristics of a new organic-inorganic hybrid membrane”,Polymer,50,553-559(2009). 18 Chrissafis,K.,Paraskevopoulos,K.M.,Tsiaoussis,I.,Bikiaris,D., “Comparative study of the effect of different nanoparticles on the mechanical properties,permeability,and thermal degradation mechanism of HDPE”,J.Appl.Polym.Sci.,112,1606-1618(2009). 19 Palkovits,R.,Althues,H.,Rumplecker,A.,Tesche,B.,Dreier,A., Holle,U.,Fink,G.,Cheng,H.,Shantz,D.F.,Kaskel,S.,“Polymerization of w/o microemulsions for the preparation of transparent SiO₂/PMMA nanocomposites”,Langmuir,21,6048-6053(2005). 20 Zhang,K.,Zheng,L. L.,Zhang,X.H.,Chen,X.,Yang,B.,“Silica-PMMA core-shell and hollow nanospheres”,Colloids and Surfaces,277,145-156(2006). 21 Ke,Y. H.,Wei,G. Y.,Wang,Y.,“Preparation,morphology and properties of nanocomposites of polyacrylamide copolymers with monodisperse silica”,Eur.Polym.J.,44,2448-2457(2008). 22 Bruce,L.J.,Chase,H.A.,“Hydrodynamics and adsorption behaviour within an expanded bed adsorption column studied using in-bed,No.4,August 2010 671 26 Zhang,X.,Liu,W. H.,Chen,Y. M.,Gong,A. J.,Chen,C. F.,Xi,F., “Self-condensing vinyl polymerization of acrylamide”,Polymer Bulletin,43,29-34(1999). 27 Savina,I. N.,Galaev,I. Y.,Mattiasson,B.,“Anion-exchange supermacroporous monolithic matrices with grafted polymer brushes of N,N-dimethylaminoethyl-methacrylate”,J.Chromatogr.A,1092, 199-205 (2005). 28 Savina,I.N.,Mattiasson,B.,Galaev,I.Y.,“Graft polymerization of acrylic acid onto macroporous polyacrylamide gel (cryogel)initiated by potassium diperiodatocuprate”,Polymer,46,9596-9603(2005). 29 Inyang,H.I.,Bae,S.,“Polyacrylamide sorption opportunity on interlayer and external pore surfaces of contaminant barrier clays”, Chemosphere,58,19-31(2005).

Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

Preparation of Supermacroporous Composite Cryogel Embedded with SiO₂ Nanoparticles

可视化

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 0

编辑推荐

Metrics

本文评价