焦化厂污染土壤中多环芳烃降解菌群解析

化工学报 ›› 2010, Vol. 61 ›› Issue (2): 477-483.

焦化厂污染土壤中多环芳烃降解菌群解析

李晓斌，孙寓姣，王红旗，丁爱中

北京师范大学水科学研究院，教育部水沙科学重点实验室

出版日期:2010-02-05 发布日期:2010-02-05

Analysis of PAHs-degrading bacteria from contaminated soil at a coking plant

LI Xiaobin，SUN Yujiao，WANG Hongqi，DING Aizhong

Online:2010-02-05 Published:2010-02-05

摘要/Abstract

摘要：

以苯并［a］芘、苯并［a］蒽、苯并［b］荧蒽、苯并［k］荧蒽、茚并［1,2,3-cd］芘5种较难降解的多环芳烃为碳源和能源，采用富集培养的方法从焦化厂污染的土壤中筛选分离得到50株PAHs降解菌。通过对其16S rRNA基因序列分析，将这些PAHs降解菌分为15个种群，分别属于Sphingomonas（鞘氨醇单胞菌属）、Methylobacterium（甲基杆菌属）、Burkholderia（伯克霍尔德氏菌属）、Rhodococcus（红球菌属）、Bradyrhizobium（慢生根瘤菌属）、Phyllobacterium（叶杆菌属）、Chryseobacterium（金黄杆菌属）、Microbacterium（微杆菌属）8个属，其中优势菌为鞘氨醇单胞菌属。纯菌株降解能力测试表明，培养12 d后，菌株3-6-12降解效果要优于其他菌株，对苯并［a］蒽、苯并［a］芘、苯并［b］荧蒽、苯并［k］荧蒽、茚并［1,2,3-cd］芘的降解率分别可达39.64%、33.52%、38.57%、25.37%、31.17%。实验结果可为多环芳烃污染土壤的生物修复提供高效的降解菌源。

关键词:

多环芳烃, 菌株筛选, 16S rRNA基因, 生物降解

Abstract:

Microbes play an important role in bioremediation technologies,the aim of this article was to investigate functional strains for soil bio-remediation.In this study benzo［a］pyrene(BaP),benzo［a］anthracene(BaA),benzo［b］fluoranthene(BbF),benzo［k］fluoranthene(BkF),indeno［1,2,3-cd］pyrene(IPY) were used as carbon and energy sources and 50 strains were isolated by steps including enrichment culture and plate cultivation from the contaminated soil at a coking plant in Beijing.The result of 16S rRNA gene sequencing analysis revealed that these strains were clustered into 15 groups and belonged to genera Sphingomonas，Methylobacterium，Burkholderia，Rhodococcus，Bradyrhizobium，Phyllobacterium，Chryseobacterium，Microbacteriumm，of which Sphingomonas was the dominant species.The highest degradation rates of BaA，BaP，BbF，BkF，IPY were 39.64%，33.52%，38.57%，25.37% and 31.17% respectively when tested with strain 3-6-12.The experimental results will be useful for bioremediation of PAHs-contaminated soil.

Key words:

多环芳烃, 菌株筛选, 16S rRNA基因, 生物降解

李晓斌, 孙寓姣, 王红旗, 丁爱中. 焦化厂污染土壤中多环芳烃降解菌群解析 [J]. 化工学报, 2010, 61(2): 477-483.

LI Xiaobin, SUN Yujiao, WANG Hongqi, DING Aizhong. Analysis of PAHs-degrading bacteria from contaminated soil at a coking plant[J]. CIESC Journal, 2010, 61(2): 477-483.

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