Abstract:

Adsorbent resin JD-1 was used for the in situ product removal (ISPR) in the pristinamycin production with S. pristinaespiralis E-71. A kinetic model for pristinamycin production and the film-pore diffusion model (FPDM) were combined together to describe the ISPR process in a 2. 5 L stirred tank bioreactor. Model parameters including pristinamycin formation rate constant, product inhibition constant, film mass transfer coefficient and pore diffusion coefficient were determined with the nonlinear least squares method. Theoretical calculations indicated that the model was in good agreement with the practical ISPR process. The effect of the amount and size of the resin on the ISPR results was studied. Experimental results showed that up to 90% of the pristinamycin produced by the fermentation could be recovered by the adsorbent resin, and that the performance got better with the decrease of resin size as well as with the increase of resin amount in a specific range. A yield of 1. 01 g·L^-1 was obtained with 95% adsorbed in situ when 70 g·L^-1 resin (R=0.195 mm) was added.