Abstract:

The flow driving system of single cell is one of the key components of flow cytometer.The method of single cell arrangement was proposed based on the digitalization of microfluids driving and controlling technology，and the driving mechanism of single cell flow was analyzed.By way of encoded digital high frequency pulse imposed on flow chamber，cells from continuous sheath fluid were split into single cell.One quartz micro flow chamber of the core of flow cytometry fabricated by sophisticated glass-processing technology was designed.The driving experiment on the lymphocytes of human peripheral blood was conducted，and real-time observation of fluidic status of single cell was performed through the fluorescence microscope.The experiment showed that the entire system was compact and easy to operate.