Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

CIESC Journal ›› 2011, Vol. 19 ›› Issue (1): 128-134.

Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

刘燕¹, 陶晶², 阎豫君², 李彬¹, 李晖², 李春^1,2

1. School of Life Science&Technology, Beijing Institute of Technology, Beijing 100081, China;
2. School of Agriculture/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Xinjiang 832003, China

收稿日期:2010-04-22 修回日期:2010-09-26 出版日期:2011-02-28 发布日期:2011-02-28
通讯作者: LI Chun, E-mail: lichun@bit.edu.cn
基金资助:
Supported by the National Natural Science Foundation of China(20776017);the Xinjiang Uygur Autonomous Region High-tech Research and Development Project(20081108);the Fok Ying Tung Education Foundation(101071);the Xinjiang Bingtuan Key Science and Technology Industry Project(2008GG24)

Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

LIU Yan¹, TAO Jing², YAN Yujun², LI Bin¹, LI Hui², LI Chun^1,2

1. School of Life Science&Technology, Beijing Institute of Technology, Beijing 100081, China;
2. School of Agriculture/Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, Shihezi University, Xinjiang 832003, China

Received:2010-04-22 Revised:2010-09-26 Online:2011-02-28 Published:2011-02-28
Supported by:
Supported by the National Natural Science Foundation of China(20776017);the Xinjiang Uygur Autonomous Region High-tech Research and Development Project(20081108);the Fok Ying Tung Education Foundation(101071);the Xinjiang Bingtuan Key Science and Technology Industry Project(2008GG24)

摘要/Abstract

摘要： The seed germination and tomato seedling tests showed that Bacillus subtilis SL-13 could promote the sprouting and seedling growth of tomato.The fresh and dry weight of tomato seedlings increased 42.86%and 18.75%,respectively.The control efficacies of the SL-13 to tomato Rhizoctonia rot were 20.65% and 35.23% in the greenhouse and field,respectively.The growth of the plant-pathogenic fungus Rhizoctonia solani was considerably inhibited in the presence of the strain SL-13 culture supernatant.The main antifungal protein was detected to be chitinase through vitro assay.The chitinase was purified with DEAE-Sepharose fast flow ion exchange column chromatography and Sephadex G-75 gel filtration for further characterization.The optimal pH and temperature for the chitinase activity were 7.0 and 50℃,respectively.It was demonstrated that the enzyme was stable at pH 5-9 and 40-60℃.70% of the enzyme activity was retained when incubated at 121℃ and 0.11 MPa for 20 min,and the enzyme was not sensitive to protease K and ultraviolet radiation.Thus it is suitable for effective biological control in relatively unstable environment.

关键词: Bacillus subtilis, growth-promoting, diseases control, antifungal chitinase, purification, Rhizoctonia solani

Abstract: The seed germination and tomato seedling tests showed that Bacillus subtilis SL-13 could promote the sprouting and seedling growth of tomato.The fresh and dry weight of tomato seedlings increased 42.86%and 18.75%,respectively.The control efficacies of the SL-13 to tomato Rhizoctonia rot were 20.65% and 35.23% in the greenhouse and field,respectively.The growth of the plant-pathogenic fungus Rhizoctonia solani was considerably inhibited in the presence of the strain SL-13 culture supernatant.The main antifungal protein was detected to be chitinase through vitro assay.The chitinase was purified with DEAE-Sepharose fast flow ion exchange column chromatography and Sephadex G-75 gel filtration for further characterization.The optimal pH and temperature for the chitinase activity were 7.0 and 50℃,respectively.It was demonstrated that the enzyme was stable at pH 5-9 and 40-60℃.70% of the enzyme activity was retained when incubated at 121℃ and 0.11 MPa for 20 min,and the enzyme was not sensitive to protease K and ultraviolet radiation.Thus it is suitable for effective biological control in relatively unstable environment.

Key words: Bacillus subtilis, growth-promoting, diseases control, antifungal chitinase, purification, Rhizoctonia solani

刘燕, 陶晶, 阎豫君, 李彬, 李晖, 李春. Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase[J]. CIESC Journal, 2011, 19(1): 128-134.

LIU Yan, TAO Jing, YAN Yujun, LI Bin, LI Hui, LI Chun. Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase[J]. , 2011, 19(1): 128-134.

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Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

Biocontrol Efficiency of Bacillus subtilis SL-13 and Characterization of an Antifungal Chitinase

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