CIESC Journal ›› 2011, Vol. 19 ›› Issue (1): 135-139.

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Enzyme-catalyzed Synthesis of Vitamin E Succinate Using a Chemically Modified Novozym-435

尹春华, 张聪, 高明   

  1. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China
  • 收稿日期:2010-07-15 修回日期:2010-11-15 出版日期:2011-02-28 发布日期:2011-02-28
  • 通讯作者: YIN Chunhua, E-mail: chyin@sina.com
  • 基金资助:
    Supported by the Fundamental Research Funds for the Central Universities and the State Key Development Program for Basic Research of China(2007CB714304)

Enzyme-catalyzed Synthesis of Vitamin E Succinate Using a Chemically Modified Novozym-435

YIN Chunhua, ZHANG Cong, GAO Ming   

  1. Department of Biological Science and Technology, School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China
  • Received:2010-07-15 Revised:2010-11-15 Online:2011-02-28 Published:2011-02-28
  • Supported by:
    Supported by the Fundamental Research Funds for the Central Universities and the State Key Development Program for Basic Research of China(2007CB714304)

摘要: Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately.We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme.The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E.Compared with the native Novozym-435,the catalytic activity of the modified novozym-435 in promoting the synthesis of vitamin E succinate was dramatically increased,with the novozym-435 modified with succinic anhydride(N435-S)as the most active catalyst.Conditions for the synthesis of vitamin E succinate were also optimized.A mixture of tert-butanol and DMSO(volume ratio of 2:3)was the most suitable medium for the reaction,whereas the appropriate molar ratio of vitamin E to succinic anhydride and reaction temperature were 1:5 and 40℃,respectively.Under these reaction conditions,the yield of vitamin E succinate reached 94.4%.N435-S could be reused for five batches.

关键词: lipase, vitamin E succinate, vitamin E, modification

Abstract: Vitamin E succinate was synthesized in organic solvents using a modified Novozym-435 as catalyst.In order to improve the catalytic performance of Novozym-435,the enzyme was modified using acetic anhydride, propionic anhydride and succinic anhydride separately.We found that both the hydrolytic activity and the thermal stability of the modified Novozym-435 were enhanced compared with the unmodified enzyme.The modified Novozym-435 catalysts were used to synthesize the succinate derivative of vitamin E.Compared with the native Novozym-435,the catalytic activity of the modified novozym-435 in promoting the synthesis of vitamin E succinate was dramatically increased,with the novozym-435 modified with succinic anhydride(N435-S)as the most active catalyst.Conditions for the synthesis of vitamin E succinate were also optimized.A mixture of tert-butanol and DMSO(volume ratio of 2:3)was the most suitable medium for the reaction,whereas the appropriate molar ratio of vitamin E to succinic anhydride and reaction temperature were 1:5 and 40℃,respectively.Under these reaction conditions,the yield of vitamin E succinate reached 94.4%.N435-S could be reused for five batches.

Key words: lipase, vitamin E succinate, vitamin E, modification