玉米浆干粉在葡萄糖和木糖混合丙酮丁醇发酵中的应用

CIESC Journal

玉米浆干粉在葡萄糖和木糖混合丙酮丁醇发酵中的应用

潘微2，熊莲1，2，彭万峰2，陈新德1，2，陈勇3

1中国科学院可再生能源与天然气水合物重点实验室，广东广州 510640；2中国科学院广州能源研究所，广东广州 510640；3中国科学院广州分院，广东广州 510640

出版日期:2011-12-05 发布日期:2012-03-02

Application of dry powder of corn steep water in the butanol fermentation by Clostridium acetobutylicum

PAN Wei2，XIONG Lian1，2，PENG Wanfeng2，CHEN Xinde1，2，CHEN Yong3

1Key Lab of Renewable Energy and Natural Gas Hydrate，Chinese Academy of Sciences，Guangzhou 510640，Guangdong，China；2Guangzhou Institute of Energy Conversion，Guangzhou 510640，Guangdong，China；3Guangzhou Branch of Chinese Academy of Sciences，Guangzhou 510640，Guangdong，China

Online:2011-12-05 Published:2012-03-02

摘要/Abstract

摘要：

为降低丙酮丁醇梭菌发酵生产丁醇的成本，研究了以玉米浆干粉为氮、磷源等营养因子的单糖培养基发酵条件。结果表明：只需添加适量的玉米浆干粉，无需添加其它营养物质，以葡萄糖或葡萄糖与木糖混合糖为原料的培养基可以满足丙酮丁醇梭菌的生长及溶剂产生所需要的营养元素，50 g/L葡萄糖-2.5 g/L玉米浆干粉培养基溶剂转化率可以达到35.14%，总溶剂产量17.57 g/L，丁醇产量11.21 g/L。30 g/L葡萄糖+20 g/L木糖-2.5 g/L玉米浆干粉溶剂转化率可以达到33.66%，总溶剂16.83 g/L，丁醇11.10 g/L，为进一步研究木质纤维素原料水解液生产丁醇提供理论指导。

Abstract:

Fermentative production of butanol by Clostridium acetobutylicum using dry powder of corn steep water（DCSW）was investigated to reduce production cost. In a batch-scale fermentation，11.21 g/L of butanol were produced by Clostridium acetobutylicum from an optimized 50g/L glucose-2.5 g/L DCSW，and 17.57 g/L of total solvents（acetone，butanol，and ethanol）was obtained. Initial fermentation in small-scale flasks with 30 g/L glucose+20 g/L xylose-2.5 g/L DCSW medium yielded 11.10 g/L of butanol and 16.83 g/L of total solvents. DCSW can serve as an economic source of nitrogen，vitamins，amino acids，minerals，and other nutrients for cellulosic biofuel butanol.

潘微2，熊莲1，2，彭万峰2，陈新德1，2，陈勇3. 玉米浆干粉在葡萄糖和木糖混合丙酮丁醇发酵中的应用[J]. CIESC Journal.

PAN Wei2，XIONG Lian1，2，PENG Wanfeng2，CHEN Xinde1，2，CHEN Yong3. Application of dry powder of corn steep water in the butanol fermentation by Clostridium acetobutylicum[J]. .