CIESC Journal

• 生物化工 • 上一篇    下一篇

一株烟腈水解酶菌株的筛选及其催化特性初步研究

何玉财,周 琼,张 跃,王龙耀,王利群,高大舟,郑 明,赵希岳,卢 彬,丁 亮   

  1. 常州大学制药与生命科学学院生物工程研究室,江苏 常州 213164
  • 出版日期:2011-12-05 发布日期:2012-03-02

Isolation and catalytic characteristics of a 3-cyanopyridine-hydrolyzing strain

HE Yucai,ZHOU Qiong,ZHANG Yue,WANG Longyao,WANG Liqun,GAO Dazhou,ZHENG Ming,ZHAO Xiyue,LU Bin,DING Liang   

  1. Laboratory of Biochemical Engineering,College of Pharmaceutical and Life Sciences,Changzhou University,Changzhou 213164,Jiangsu,China
  • Online:2011-12-05 Published:2012-03-02

摘要:

利用富集培养技术筛选获得一株高活性烟腈水解菌株Rhodococcus sp. CCZU10-1。经研究,最适产酶培养条件和催化反应条件为:碳源为葡萄糖(10 g/L),氮源为酵母蛋白胨(10 g/L)与酵母膏(5 g/L),诱导剂ε-己内酰胺(2.0 mmol/L),培养温度为30 ,初始培养pH值为7.0,最适催化反应温度和pH值分别为30 7.0。分批补料烟腈10批,烟酸的累计浓度为927 mmol/L

Abstract:

Rhodococcus sp. CCZU10-1 has been isolated from soil samples with high nitrilase activity against 3-cyanopyridine using the enrichment culture technique. The preferred carbon sourcesnitrogen sourcesand inducer were glucose10 g/L),a composite of peptone10 g/Land yeast extract5 g/L),and ε-caprolactam2.0 mmol/L),respectively. The optimal reaction temperature and pH for 3-cyanopyridine-hydrolyzing activity were 30 and pH 7.0respectively. Using fed-batch methoda total of 927 mmol/L nicotinic acid has been accumulated in the reaction mixture after the 10th feed.