Abstract:

Synthesis of chiral drugs and intermediates catalyzed by enzyme has high efficiency，energy saving and environment-friendliness advantages. This paper reviews the solvent effect on the enzymatic reaction in non-aqueous media with lipases，namely，the activity and enantioselectivity of enzyme adjusted by solvent system to improve the efficiency of catalytic reaction. The kinetically controlled synthesis of β-lactam antibiotics with penicillin G acylase was introduced in different solvent systems，including the organic-water miscible solvents，organic-water immiscible solvents，reverse micelles and aqueous two-phase system. The yield of kinetically controlled synthesis of β-lactam antibiotics and synthesis/hydrolysis ratio（S/H）could be enhanced by designing an effective solvent system to better utilize the substrates.