摘要：

在10 L发酵罐、控制甲醇浓度的条件下，考察了诱导温度对毕赤酵母发酵生产猪α-干扰素（pIFN-α）性能的影响。结果表明，温度对pIFN-α表达的影响明显，20℃低温有利于pIFN-α的表达，该条件下pIFN-α最高活性可达1.5×106 IU·ml^-1，是30℃传统诱导生产时最高活性（1.0

关键词: 毕赤酵母；猪αFONT-FAMILY: Calibri,sans-serif, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体, mso-hansi-font-family: 宋体">-FONT-FAMILY: 宋体, mso-bidi-font-size: 11.0pt, mso-ascii-font-family: Calibri, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">干扰素；诱导；代谢分析

Abstract:

The effect of induction temperatures on porcine interferon-α(pIFN-α）production byPichia pastoris was investigated in a 10 L bioreactor with methanol concentration controlled at a specific level. The results indicated that a low temperature was obviously beneficial for pIFN-α expression, and the highest pIFN-α antiviral activity reached a level of 1.5×10⁶ IU·ml^-1 when induction was made at 20℃, that was 150-folds of the maximal value obtained with the traditional induction strategy at 30℃.On-line multi-variable measurement of the fermentative state variables and metabolic analysis showed that cell metabolic activity and specific methanol consumption rate (0.036 h^-1）could be higher with induction at 20

Key words: 毕赤酵母；猪αFONT-FAMILY: Calibri,sans-serif, mso-bidi-font-size: 11.0pt, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA, mso-fareast-font-family: 宋体, mso-hansi-font-family: 宋体">-FONT-FAMILY: 宋体, mso-bidi-font-size: 11.0pt, mso-ascii-font-family: Calibri, mso-bidi-font-family: 宋体, mso-font-kerning: 1.0pt, mso-ansi-language: EN-US, mso-fareast-language: ZH-CN, mso-bidi-language: AR-SA">干扰素；诱导；代谢分析