Abstract:

In order to eliminate the bacteria in inoculating sludge, which consume hydrogen, and to speed start-up as well as to rise efficiency of hydrogen production, effect of pretreatment temperatures of 65, 80, 95, 110 and 121℃ for 30 min on hydrogen production from glucose was investigated in a batch cultures, with anaerobic granular sludge come from workshop treating wastewater of beer factory.The results come from fermentation showed that the amount of hydrogen produced, at conditions of initial pH=7.0, glucose concentration 10000 mg·L^-1 and amount of inoculating sludge 2 g MLVSS·L^-1, was less for untreated sludge than pretreated one.For anaerobic granular sludge treated at 95℃, the maximum accumulative hydrogen production in 120 h, 133.47 ml, was obtained, and equal to hydrogen yields of 1.40 mol·mol^-1 glucose, and specific hydrogen production of 15.93 mmol H₂·(g MLVSS)^-1. Although hydrogen production was varied for different pretreatment temperature, butyrate and acetate were dominated in soluble liquid products for all glucose fermentation tests, and their account was about 80% of the total volatile fatty acids and ethanol.