关键词: 疏水性电荷诱导层析, 免疫球蛋白, IgY, 鸡血浆

Abstract:

Hydrophobic charge induction chromatography(HCIC)was used to separate immunoglobulin IgY from chicken plasma.The plasma was pretreated with caprylic acid to precipitate some impurities, and the supernatant was used directly as the feedstock for separation and purification of immunoglobulin IgY by HCIC.Two resins, commercial MEP HyperCel and homemade BestaroseDVSMMI, were used as HCIC medium and investigated.Operation conditions were optimized, including pH value of sample loading and elution, as well as sample loading volume.It was found that BestaroseDVSMMI with 2mercapto1methylimidazole as the ligand was better than MEP HyperCel.The results indicated that IgY could be separated with high purity(above 92% with HPLC analysis)and yield about 95%.The mechanism of HCIC was discussed based on comparison of ligand structure and surface potential.The results demonstrated that HCIC can be efficiently used for IgY purification from chicken plasma, which can be a new approach for the immunoglobulin separation from the plasma.

Key words: 疏水性电荷诱导层析, 免疫球蛋白, IgY, 鸡血浆