关键词: 钝齿棒杆菌, 供氧差异, 蛋白质组, 基质辅助激光解析飞行时间质谱

Abstract:

Dissolved oxygen level directly affects the synthesis of arginine by Corynebacterium crenatum(C.crenatum).Proteomic approaches including twodimensional electrophoresis(2DE)and matrixassisted laserdesorption/ionization timeofflight mass spectrometry(MALDITOFMS)were used to analyze differential expression of proteins in C.crenatum in different oxygen supply conditions.The results showed that most of the isoelectric points of the proteins on the 2DE gels distributed from 4 to 7.Thirtytwo different protein points were detected in the pH 4—7 gels and characterized by MALDITOFMS as well as peptide mass fingerprintings.Using matrixscience database, 29 protein points were identified, and they represented 27 proteins.Database research showed that the proteins performed different functions in carbohydrate metabolism, amino acid metabolism and energy metabolism.Most of the differentially expressed proteins concentrated in the pentose phosphate pathway, TCA cycle and the urea cycle.The results showed that in the high oxygen supply model, energy metabolism was significantly increased, and the oxidative stress was balanced because of the increase of pentose phosphate pathway.Meanwhile, the activation of TCA cycle and urea cycle were beneficial to the synthesis of arginine.

Key words: 钝齿棒杆菌, 供氧差异, 蛋白质组, 基质辅助激光解析飞行时间质谱