大团囊虫草菌胞内多糖发酵优化和抗氧化活性研究

CIESC Journal ›› 2012, Vol. 20 ›› Issue (2): 294-301.

大团囊虫草菌胞内多糖发酵优化和抗氧化活性研究

许勤勤, 刘振华, 孙一晟, 丁忠杰, 吕龙贤, 李永泉

College of Life Sciences, Zhejiang University, Hangzhou 310058, China

收稿日期:2011-05-11 出版日期:2012-04-28 发布日期:2011-08-26

Optimization for production of intracellular polysaccharide from Cordyceps ophioglossoides L2 in submerged culture and its antioxidant activities in vitro

XU Qin-Qin, LIU Zhen-Hua, SUN Yi-Sheng, DING Zhong-Jie, LV Long-Xian , LI Yong-Quan

College of Life Sciences, Zhejiang University, Hangzhou 310058, China

Received:2011-05-11 Online:2012-04-28 Published:2011-08-26

摘要/Abstract

摘要： Cordyceps ophioglossoides is a valuable traditional medicinal material. We have found that intracellular polysaccharide (IPS) is the major biologically active ingredient in Cordyceps ophioglossoides. This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides. The optimal medium for IPS production consists of glucose 54.50 g•L?1, yeast powder 25.50 g•L?1, NaH2PO4 0.4 g•L?1 and K2HPO4 0.4 g•L?1. The suggested culture conditions are 24 °C, initial pH 4.5 with a rotary speed of 120 r•min?1 for 168 h. The yield of IPS is 737.93 mg•L?1, which is 50% higher than the yield under the conditions prior to optimization. The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay. The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

关键词: antioxidant activity, Cordyceps ophioglossoides L2, intracellular polysaccharide, submerged culture, optimization

Abstract: Cordyceps ophioglossoides is a valuable traditional medicinal material. We have found that intracellular polysaccharide (IPS) is the major biologically active ingredient in Cordyceps ophioglossoides. This study is the first time to optimize the yield of IPS from Cordyceps ophioglossoides. The optimal medium for IPS production consists of glucose 54.50 g•L?1, yeast powder 25.50 g•L?1, NaH2PO4 0.4 g•L?1 and K2HPO4 0.4 g•L?1. The suggested culture conditions are 24 °C, initial pH 4.5 with a rotary speed of 120 r•min?1 for 168 h. The yield of IPS is 737.93 mg•L?1, which is 50% higher than the yield under the conditions prior to optimization. The anti-oxidative activities of IPS in Cordyceps ophioglossoides L2 are also characterized using various in vitro assay. The anti-oxidative activity may explain the reason why IPS from Cordyceps ophioglossoides can be used to fight against neurodegenerative dis-eases and menopausal symptoms.

Key words: antioxidant activity, Cordyceps ophioglossoides L2, intracellular polysaccharide, submerged culture, optimization

许勤勤, 刘振华, 孙一晟, 丁忠杰, 吕龙贤, 李永泉. 大团囊虫草菌胞内多糖发酵优化和抗氧化活性研究[J]. CIESC Journal, 2012, 20(2): 294-301.

XU Qin-Qin, LIU Zhen-Hua, SUN Yi-Sheng, DING Zhong-Jie, LV Long-Xian , LI Yong-Quan. Optimization for production of intracellular polysaccharide from Cordyceps ophioglossoides L2 in submerged culture and its antioxidant activities in vitro[J]. , 2012, 20(2): 294-301.

[1]	费正顺, 胡斌, 叶鲁彬, 梁军. 基于ARX-NNPLS模型的优化策略及在聚合物牌号切换过程中的应用[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 971-979.
[2]	罗剑飞, 林炜铁, 蔡小龙, 李敬源. 基于神经网络与遗传算法结合的硝化细菌发酵培养基优化[J]. Chinese Journal of Chemical Engineering, 2012, 20(5): 950-957.
[3]	吴新杰, 崔春阳, 胡晟, 李志宏, 吴成东. 基于粒子群优化算法和非线性盲源信号分离测量两相流速度[J]. CIESC Journal, 2012, 20(2): 346-351.
[4]	郑广俭, 崔学民, 张伟鹏, 童张法, 李峰. 具有固定硅铝比的Al₂O₃-2SiO₂纳米颗粒制备：过程优化与粉体转化[J]. CIESC Journal, 2012, 20(2): 312-318.
[5]	焦云强, 苏宏业, 廖祖维, 侯卫锋. Modeling and Multi-objective Optimization of Refinery Hydrogen Network[J]. CIESC Journal, 2011, 19(6): 990-998.
[6]	胡纯铿, 秦晴, 高培培. Medium Optimization for Improved Ethanol Production in Very High Gravity Fermentation[J]. CIESC Journal, 2011, 19(6): 1017-1022.
[7]	Andreas Harwardt, Korbinian Kraemer, Bettina Rüngeler, Wolfgang Marquardt. Conceptual Design of a Butyl-levulinate Reactive Distillation Process by Incremental Refinement[J]. CIESC Journal, 2011, 19(3): 371-379.
[8]	Ivana Karabegović, Milena Nikolova, Dragan Veličković, Sаša Stojičević, Vlada Veljković, Miodrag Lazić. Comparison of Antioxidant and Antimicrobial Activities of Methanolic Extracts of the Artemisia sp. Recovered by Different Extraction Techniques[J]. CIESC Journal, 2011, 19(3): 504-511.
[9]	Ignacio E. Grossmann, Mariano Martín. Energy and Water Optimization in Biofuel Plants[J]. CIESC Journal, 2010, 18(6): 914-922.
[10]	摆亮, 江永亨, 黄德先, 刘先广. A Novel Scheduling Strategy for Crude Oil Blending[J]. CIESC Journal, 2010, 18(5): 777-786.
[11]	王建林, 薛尧予, 于涛, 赵利强. Run-to-run Optimization for Fed-batch Fermentation Process with Swarm Energy Conservation Particle Swarm Optimization Algorithm[J]. CIESC Journal, 2010, 18(5): 787-794.
[12]	李政, 邓利, 鲁吉珂, 郭小雷, 杨自信, 谭天伟. Enzymatic Synthesis of Fatty Acid Methyl Esters from Crude Rice Bran Oil with Immobilized Candida sp. 99-125[J]. CIESC Journal, 2010, 18(5): 870-875.
[13]	Juan A. Lazzús. Prediction of Flash Point Temperature of Organic Compounds Using a Hybrid Method of Group Contribution+Neural Network+Particle Swarm Optimization[J]. CIESC Journal, 2010, 18(5): 817-823.
[14]	肖丰, 都健, 刘琳琳, 栾国颜, 姚平经. Simultaneous Optimization of Synthesis and Scheduling of Cleaning in Flexible Heat Exchanger Networks[J]. CIESC Journal, 2010, 18(3): 402-411.
[15]	袁海娜, 姚善泾, 尤玉如, 肖功年, 尤琦. Antioxidant Activity of Isothiocyanate Extracts from Broccoli[J]. CIESC Journal, 2010, 18(2): 312-321.