关键词: 阿魏酸酯酶, 木聚糖酶, 麦糟, 生物降解

Abstract: The optimal fermentation time of Penicillium citrinum feruloyl esterase（PcFAE）was 60 h and its activity was 40.8 mU/mL when the Brewers’ Spent Grain（BSG）was used as the sole carbon source for Penicillium citrinum. One gram of alcohol-insoluble residue（AIR）of Brewers’spent grain（BSG）was processed by the solution of crude xylanase（XG180）（47.5 U/mL，15 mL）for 12 hours at the condition of the material-liquid ratio 1∶30（W/V），pH 5.0 and 45 ℃，respectively. And then the solution of crude PcFAE（40.8 mU/mL）was added and the sample was processed for another 12 h. The maximum release rate of Ferulic acid（FA）was 54.1% and the release amount of xylooligosaccharide（XOS）was 161 mg/g（BSG-AIR），respectively. The results of our experiments showed that the release rate of FA and XOS from BSG-AIR increased significantly as the PcFAE could coordinate with the xylanase and enzymolysis process was also conducive to the degradation of BSG.

Key words: feruloyl esterases；xylanase；brewers&rsquo, spent grain；biodegradation