关键词: 混合模式层析, 2-巯基咪唑, 二乙烯基砜活化, 免疫球蛋白, 牛初乳

Abstract: A new mixed-mode absorbent，named B-DVS-MI，with functional ligand 2-mercaptoimidazole was prepared and used for separation of immunoglobulin from bovine colostrums.There could exist hydrophobic，electrostatic and thiophilic interactions between B-DVS-MI and the adsorbate.Divinylsulfone activation and ligand coupling processes on the agarose beads were employed and optimized，resulting in activation density of 100 μmol·ml-1 and ligand density of 65 μmol·ml-1.The adsorption isotherm and kinetics for B-DVS-MI - bovine colostrum immunoglobulin system were measured and analyzed.The results showed that new adsorbent was of high capacity of immunoglobulin［127.6 mg·(g gel)-1］，and of typical salt-independent properties.Its adsorption behaviors were significantly influenced by pH，so，with pH adjustment effective adsorption and elution could be achieved.The chromatographic conditions were optimized to separate effectively immunoglobulin from bovine colostrum powder，and product purity up to 95%.As a conclusion，bovine colostrum immunoglobulin could be effectively separated by mixed-mode adsorption chromatography，which could be a new way for comprehensive utilization of bovine colostrums.

Key words: mixed-mode chromatography, 2-mercaptoimidazole, divinylsulfone activation, immunoglobulin, bovine colostrums