关键词: 手性化合物, 辅酶再生体系, 酶耦合反应, 细胞耦合, 内源性辅酶再生酶

Abstract: Cofactor regeneration and repeated utilization showed important effect on the preparation of chiral compound by the redox biocatalysts. Cofactor regenerated by enzymatic catalysis had high efficiency and specificity，such cofactor regenerated system include single cell in vivo cofactor regenerated system，double cell coupling system and in vitro enzyme coupling system due to their catalysis circumstance. The construct methods of different cofactor regenerated system were presented particularly in this paper. The possible issues in practical application of these cofactor regenerated system were especially pointed out and the resolve methods were afforded，such as the solution of low water-soluble organic substrate by ionic liquid，the subsistence of micro-molecule cofactor by covalent immobilization and the utilization of endogenous cofactor regenerated enzyme in cell free extract. The advantage and limitation of different cofactor regenerated systems that applied in industry catalysis were also commented，more attention should be focused on the repeated and multi-batches utilization of the cofactor regenerated system.

Key words: chiral compound, cofactor regenerated system, enzyme coupling reaction, cell coupling, endogenous cofactor regenerated enzyme