一株烟腈水解酶菌株的筛选及其催化特性初步研究

CIESC Journal

一株烟腈水解酶菌株的筛选及其催化特性初步研究

何玉财，周琼，张跃，王龙耀，王利群，高大舟，郑明，赵希岳，卢彬，丁亮

常州大学制药与生命科学学院生物工程研究室，江苏常州 213164

出版日期:2011-12-05 发布日期:2012-03-02

Isolation and catalytic characteristics of a 3-cyanopyridine-hydrolyzing strain

HE Yucai，ZHOU Qiong，ZHANG Yue，WANG Longyao，WANG Liqun，GAO Dazhou，ZHENG Ming，ZHAO Xiyue，LU Bin，DING Liang

Laboratory of Biochemical Engineering，College of Pharmaceutical and Life Sciences，Changzhou University，Changzhou 213164，Jiangsu，China

Online:2011-12-05 Published:2012-03-02

摘要/Abstract

摘要：

利用富集培养技术筛选获得一株高活性烟腈水解菌株Rhodococcus sp. CCZU10-1。经研究，最适产酶培养条件和催化反应条件为：碳源为葡萄糖（10 g/L），氮源为酵母蛋白胨（10 g/L）与酵母膏（5 g/L），诱导剂ε-己内酰胺（2.0 mmol/L），培养温度为30 ℃，初始培养pH值为7.0，最适催化反应温度和pH值分别为30 ℃和7.0。分批补料烟腈10批，烟酸的累计浓度为927 mmol/L。

Abstract:

Rhodococcus sp. CCZU10-1 has been isolated from soil samples with high nitrilase activity against 3-cyanopyridine using the enrichment culture technique. The preferred carbon sources，nitrogen sources，and inducer were glucose（10 g/L），a composite of peptone（10 g/L）and yeast extract（5 g/L），and ε-caprolactam（2.0 mmol/L），respectively. The optimal reaction temperature and pH for 3-cyanopyridine-hydrolyzing activity were 30 ℃ and pH 7.0，respectively. Using fed-batch method，a total of 927 mmol/L nicotinic acid has been accumulated in the reaction mixture after the 10th feed.

何玉财，周琼，张跃，王龙耀，王利群，高大舟，郑明，赵希岳，卢彬，丁亮. 一株烟腈水解酶菌株的筛选及其催化特性初步研究[J]. CIESC Journal.

HE Yucai，ZHOU Qiong，ZHANG Yue，WANG Longyao，WANG Liqun，GAO Dazhou，ZHENG Ming，ZHAO Xiyue，LU Bin，DING Liang . Isolation and catalytic characteristics of a 3-cyanopyridine-hydrolyzing strain[J]. .