CIESC Journal

• Volume 3 • 上一篇    下一篇

EFFECTS OF TURBULENT SHEAR STRESS ON THE GROWTH OF SUSPENDED PLANT CELLS IN A BUBBLE COLUMN REACTOR

杨军政; 胡宗定; 王一平   

  1. Department of Chemical Engineering, Tianjin University, Tianjin 300072, China
  • 收稿日期:1993-09-01 修回日期:1994-03-08 出版日期:1995-06-28 发布日期:1995-06-28
  • 通讯作者: 杨军政

EFFECTS OF TURBULENT SHEAR STRESS ON THE GROWTH OF SUSPENDED PLANT CELLS IN A BUBBLE COLUMN REACTOR

YANG Junzheng; HU Zongding; WANG Yiping   

  1. Department of Chemical Engineering, Tianjin University, Tianjin 300072, China
  • Received:1993-09-01 Revised:1994-03-08 Online:1995-06-28 Published:1995-06-28
  • Contact: YANG Junzheng

摘要: In this paper,effects of increasing the height of column,bubble size and gas flow rate onthe growth of suspended Catharanthus roseus plant cells in a 1.5 litre bubble column were studied.Statistical characteristics of turbulence such as auto-correlation function,and auto-power spectral den-sity function of the nuctuating liquid velocity were measured in a bubble column employing pseudo-cell-granulated agar.Calculations of turbulent shear stress were carried out.From these studies it is sug-gested that in plant cell suspension culture sparged with large bubbles.turbulent shear stress is prob-ably one of the most important reasons for the inhibited cell growth and cell damage.Larger bubblesor higher gas flow rates lead to the increase in shear stress and are more detrimental to plant cells.

关键词: Catharanthus roseus;shear stress;plant cell;bubble column

Abstract: In this paper,effects of increasing the height of column,bubble size and gas flow rate onthe growth of suspended Catharanthus roseus plant cells in a 1.5 litre bubble column were studied.Statistical characteristics of turbulence such as auto-correlation function,and auto-power spectral den-sity function of the nuctuating liquid velocity were measured in a bubble column employing pseudo-cell-granulated agar.Calculations of turbulent shear stress were carried out.From these studies it is sug-gested that in plant cell suspension culture sparged with large bubbles.turbulent shear stress is prob-ably one of the most important reasons for the inhibited cell growth and cell damage.Larger bubblesor higher gas flow rates lead to the increase in shear stress and are more detrimental to plant cells.

Key words: Catharanthus roseus, shear stress, plant cell, bubble column