对比分析进水基质浓度对乙醇型和丁酸型发酵制氢系统的影响

doi:10.11949/j.issn.0438-1157.20150854

化工学报 ›› 2015, Vol. 66 ›› Issue (12): 5111-5118.DOI: 10.11949/j.issn.0438-1157.20150854

对比分析进水基质浓度对乙醇型和丁酸型发酵制氢系统的影响

昌盛¹, 刘枫²

1 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012;
2 哈尔滨工业大学市政环境工程学院, 黑龙江哈尔滨 150090

收稿日期:2015-06-08 修回日期:2015-09-23 出版日期:2015-12-05 发布日期:2015-12-05
通讯作者: 昌盛
基金资助:
国家自然科学基金项目(51508539,51178316);国家水体污染控制与治理重大专项(2014ZX07405-001)。

Comparison of impact of influent substrate concentration on fermentative hydrogen production by ethanol-type and butyric-type fermentation

CHANG Sheng¹, LIU Feng²

1 State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2 School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, Heilongjiang, China

Received:2015-06-08 Revised:2015-09-23 Online:2015-12-05 Published:2015-12-05
Supported by:
supported by the National Natural Science Foundation of China (51508539, 51178136) and the National Major Projects of Water Pollution Control and Treatment (2014ZX07405-001).

摘要/Abstract

摘要：

以糖蜜废水为基质,将两套厌氧接触式发酵制氢反应器(ACR)出水pH分别控制在4.5～5.0、5.5～6.0的水平,通过逐级提升进水COD浓度方式,系统对比分析基质浓度对乙醇型和丁酸型发酵制氢系统的影响。结果显示,对于乙醇型发酵制氢系统而言,当HRT 6 h,进水COD从5000逐步提升至12000 mg·L^-1时,反应器的产氢效能逐步得到增强,但当COD进一步提升至15000 mg·L^-1时,底物反馈抑制作用开始显现,因而在进水COD为12000 mg·L^-1时,ACR产氢性能最佳,系统的产氢速率、污泥比产氢速率和单位基质氢气转化率分别为68.8 L·d^-1、744.5 ml H₂·(g VSS·d)^-1、2.3 mol H₂·(mol葡萄糖)^-1。对于丁酸型发酵制氢系统而言,当HRT 8 h,在进水COD从5000提升至20000 mg·L^-1过程中,ACR产氢效能总体呈下降趋势,在进水COD为5000 mg·L^-1时,系统的污泥比产氢速率和单位基质氢气转化率最大,分别为159.6 ml H₂·(g VSS·d)^-1、1.0 mol H₂·(mol葡萄糖)^-1。研究结果表明,在进水COD为500～20000 mg·L^-1的运行中,ACR乙醇型发酵系统的产氢效能优于丁酸型发酵制氢系统。

关键词: 厌氧接触式反应器, 进水基质浓度, 化学需氧量(COD), 发酵制氢, 发酵类型

Abstract:

In the present study, the anaerobic contact reactor (ACR) was used to produce hydrogen from diluted molasses by anaerobic fermentation, and an ethanol-type fermentation and butyric-type fermentation were established by keeping the effluent pH of the two reactors at 4.5-5.0 and 5.5-6.0, respectively. And then the comparison of the impact of influent substrate concentration on the performance of the above two reactors was investigated. For the ethanol-type fermentation system, the fermentative hydrogen-producing efficiency was enhanced when the influent chemical oxygen demanding (COD) was increased from 5000 to 12000 mg·L^-1 under the HRT of 6 h, whereas, the feedback inhibition was turn to be functioned as the influent COD was increased to 15000 mg·L^-1. It was found that the hydrogen production rate (HPR), specific hydrogen rate of sludge (SHPR) and hydrogen yield (HY) was peaked at 68.8 L·d^-1, 744.5 ml H₂·(g VSS·d)^-1 and 2.3 mol H₂·(mol glucose)^-1, respectively, when the influent COD was 12000 mg·L^-1. For the butyric-type fermentation system, the hydrogen producing efficiency was decreased on the whole as the influent COD was increased from 5000 to 20000 mg·L^-1under the HRT of 8 h. It also showed that the maximum value of SHPR and HY was 159.6 ml H₂·(g VSS·d)^-1 and 1.0 mol H₂·(mol glucose)^-1, respectively, when the influent COD was 5000 mg·L^-1. It indicated that the hydrogen-producting capability of ethanol-type fermentation was better than that of butyric-type fermentation.

Key words: anaerobic contact reactor (ACR), influent substrate concentration, chemical oxygen demanding (COD), fermentative hydrogen production, fermentation type

中图分类号:

X703

昌盛, 刘枫. 对比分析进水基质浓度对乙醇型和丁酸型发酵制氢系统的影响[J]. 化工学报, 2015, 66(12): 5111-5118.

CHANG Sheng, LIU Feng. Comparison of impact of influent substrate concentration on fermentative hydrogen production by ethanol-type and butyric-type fermentation[J]. CIESC Journal, 2015, 66(12): 5111-5118.

参考文献 25

[1]	Lee H S, Rittmann B E. Evaluation of metabolism using stoichiometry in fermentative biohydrogen [J]. Biotechnology and Bioengineering, 2009, 102 (3): 749-758.
[2]	Ren N Q, Chua H, Chan S Y, Tsang Y F, Wang Y J, Sin N. Assessing optimal fermentation type for bio-hydrogen production in continuous- flow acidogenic reactors [J]. Bioresource Technology, 2007, 98 (9): 1774-1780.
[3]	Ren Nanqi, Li Jianzheng, Li Baikun, Wang Yong, Liu Shirui. Biohydrogen production from molasses by anaerobic fermentation with a pilot-scale bioreactor system [J]. International Journal of Hydrogen Energy, 2006, 31 (15): 2147-2157.
[4]	Chang Sheng (昌盛). Operation control and capability of fermentative hydrogen production of anaerobic contact reactor[D]. Harbin: Harbin Institute of Technology, 2012.
[5]	Chang Sheng (昌盛), Li Jianzheng (李建政), Fu Qing (付青), Zhao Xingru (赵兴茹), Zheng Guochen (郑国臣). Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations [J]. CIESC Journal (化工学报), 2015, 66 (3): 1156-1162.
[6]	Ramachandran U, Wrana N, Cicek N, et al. Hydrogen production and end-product synthesis patterns by Clostridium termitidis strain CT1112 in batch fermentation cultures with cellobiose or alpha-cellulose [J]. International Journal of Hydrogen Energy, 2008, 33 (23): 7006-7012.
[7]	Bakonyi P, Nemestóthy N, Simon V, Bélafi-Bakó K. Review on the start-up experiences of continuous fermentative hydrogen producing bioreactors [J]. Renewable and Sustainable Energy Reviews, 2014, 40: 806-813.
[8]	Hung C H, Chang Y T, Chang Y J. Roles of microorganisms other than Clostridium and Enterobacter in anaerobic fermentative biohydrogen production systems: a review [J]. Bioresource Technology, 2011, 102 (18): 8437-8444.
[9]	Li H Z, Li B K, Zhu G F, et al. Hydrogen production from diluted molasses by anaerobic hydrogen producing bacteria in an anaerobic baffled reactor (ABR) [J]. International Journal of Hydrogen Energy, 2007, 32 (15): 3274-3283.
[10]	Xing D F, Ren N Q, Li Q B. Ethanoligenens harbinense gen. nov., sp nov., isolated from molasses wastewater [J]. International Journal of Systematic and Evolutionary Microbiology, 2006, 56 (4): 755-760.
[11]	Guo W Q, Ren N Q, Wang X J, et al. Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor [J]. International Journal of Hydrogen Energy, 2008, 33 (19): 4981-4988.
[12]	Ren Nanqi, Xing Defeng, Rittmann B E, Zhao Lihua, Xie Tianhui, Zhao Xin. Microbial community structure of ethanol type fermentation in bio-hydrogen production [J]. Environmental Microbiology, 2007, 9 (5): 1112-1125.
[13]	Li Jianzheng, Zheng Guochen, He Junguo, Chang Sheng, Qin Zhi. Hydrogen-producing capability of anaerobic activated sludge in three types of fermentations in a continuous stirred-tank reactor [J]. Biotechnology Advances, 2009, 27 (5): 573-577.
[14]	Luo G, Karakashev D, Xie L, Zhou Q, Angelidaki I. Long-term effect of inoculum pretreatment on fermentative hydrogen production by repeated batch cultivations: homoacetogenesis and methanogenesis as competitors to hydrogen production [J]. Biotechnology and Bioengineering, 2011, 108 (8): 1816-1827.
[15]	Ren N Q, Tang J, Liu B F, et al. Biological hydrogen production in continuous stirred tank reactor systems with suspended and attached microbial growth [J]. International Journal of Hydrogen Energy, 2010, 35 (7): 2807-2813.
[16]	Pakarinen O, Kaparaju P, Rintala J. The effect of organic loading rate and retention time on hydrogen production from a methanogenic CSTR [J]. Bioresource Technology, 2011,102: 8952-8957.
[17]	Ferraz J A D N, Wenzel J, Etchebehere C, Zaiat M. Effect of organic loading rate on hydrogen production from sugarcane vinasse in thermophilic acidogenic packed bed reactors [J]. International Journal of Hydrogen Energy, 2014, 39 (30): 16852-16862.
[18]	Hafez H, Nakhla G, El Naggar M H, Elbeshbishy E, Baghchehsaraee B. Effect of organic loading rate on a novel hydrogen bioreactor [J]. International Journal of Hydrogen Energy, 2010, 35: 81-92.
[19]	Ferraz J A D N, Zaiat M, Gupta M, Elbeshbishy E, Hafez H, Nakhla G. Impact of organic loading rate on biohydrogen production in an up-flow anaerobic packed bed reactor (UAnPBR) [J]. Bioresource Technology, 2014, 164: 371-379.
[20]	Spagni A, Casu S, Farina R. Effect of the organic loading rate on biogas composition in continuous fermentative hydrogen production [J]. Journal of Environtal Science and Health, Part A: Toxic/Hazardous Substances and Environmental Engineering, 2010, 45 (12): 1475-1481.
[21]	Li Jianzheng (李建政), Chang Sheng (昌盛). Anaerobic contact digester reactor [P]: CN, 200710144460. 2008-05-14.
[22]	National Environmental Protection Agency (国家环保局). Water and Exhausted Water Monitoring Analysis Method (水和废水监测分析方法) [M]. 3rd ed. Beijing: Chinese Environment Science Press, 1997: 102, 106, 107.
[23]	Wang J L, Wan W. Factors influencing fermentative hydrogen production: a review [J]. International Journal of Hydrogen Energy, 2009, 34 (2): 799-811.
[24]	Kyazze G, Martinez-Perez N, Dinsdale R, Premier G C, Hawkes F R, Guwy A J, Hawkes D L. Influence of substrate concentration on the stability and yield of continuous biohydrogen production [J]. Biotechnology and Bioengineering, 2006, 93 (5): 971-979.
[25]	Guo W Q, Ren N Q, Wang X J, Xiang W S, Ding J, You Y, Liu B F. Optimization of culture conditions for hydrogen production by Ethanoligenens harbinense B49 using response surface methodology [J]. Bioresource Technology, 2009, 100 (3): 1192-1196.

对比分析进水基质浓度对乙醇型和丁酸型发酵制氢系统的影响

Comparison of impact of influent substrate concentration on fermentative hydrogen production by ethanol-type and butyric-type fermentation

PDF (PC)

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献 25

相关文章 4

编辑推荐

Metrics

本文评价

[1]	昌盛, 李建政, 付青, 赵兴茹, 郑国臣. ACR在不同进水COD浓度下的产氢性能与菌群结构[J]. 化工学报, 2015, 66(3): 1156-1162.
[2]	秦娟, 辛寅昌, 马德华. 微乳液的油水分离和机理探讨及应用[J]. 化工学报, 2013, 64(5): 1797-1802.
[3]	秦娟辛寅昌马德华. 微乳液的油水分离和机理探讨及应用[J]. CIESC Journal, 2013, 64(5): 0-0.
[4]	李建政,于泽,昌盛,苏晓煜. CSTR和ACR丁酸型发酵制氢系统的运行特性比较[J]. 化工学报, 2012, 63(5): 1551-1557.