ACR在不同进水COD浓度下的产氢性能与菌群结构

doi:10.11949/j.issn.0438-1157.20141345

化工学报 ›› 2015, Vol. 66 ›› Issue (3): 1156-1162.DOI: 10.11949/j.issn.0438-1157.20141345

ACR在不同进水COD浓度下的产氢性能与菌群结构

昌盛^1,2, 李建政², 付青¹, 赵兴茹¹, 郑国臣²

1 中国环境科学研究院环境基准与风险评估国家重点实验室, 北京 100012;
2 哈尔滨工业大学市政环境工程学院, 黑龙江哈尔滨 150090

收稿日期:2014-09-03 修回日期:2014-11-03 出版日期:2015-03-05 发布日期:2015-03-05
通讯作者: 昌盛
基金资助:
国家自然科学基金项目(51178316);国家水体污染控制与治理重大专项(2014ZX07405-001);国家环保公益项目(201409029)。

Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations

CHANG Sheng^1,2, LI Jianzheng², FU Qing¹, ZHAO Xingru¹, ZHENG Guochen²

1 State Environmental Protection Key Laboratory of Drinking Water Source Protection, Chinese Research Academy of Environmental Sciences, Beijing 100012, China;
2 School of Municipal and Environmental Engineering, Harbin Institute of Technology, Harbin 150090, Heilongjiang, China

Received:2014-09-03 Revised:2014-11-03 Online:2015-03-05 Published:2015-03-05
Supported by:
supported by the National Natural Science Foundation of China(51178136), the National Major Projects of Water Pollution Control and Treatment (2014ZX07405-001) and the National Environmental Charity Project (201409029).

摘要/Abstract

摘要：

以稀释糖蜜为底物,通过厌氧接触式发酵制氢反应器(ACR) 的启动和运行,考察了ACR在不同进水COD浓度下的运行特性。结果表明,当HRT= 6 h,进水COD浓度从 7000 mg·L^-1提升至11000 mg·L^-1时,反应器仍能稳定运行,并维持乙醇型发酵类型。随着底物浓度的增加,系统的比产氢速率从COD 7000 mg·L^-1时的2.43 m³·(m³·d)^-1提高到COD11000 mg·L^-1时的3.51 m³·(m³·d)^-1,而活性污泥的比产氢速率在COD 为9000 mg·L^-1时最高,为10.71 mol H₂·(kg VSS·d)^-1。聚合酶链式反应-变性梯度凝胶电泳(PCR-DGGE)分析结果表明,产氢发酵产乙醇菌群为ACR系统中的主要产氢功能菌群,且随着进水COD浓度的增加,以Ethanoligenens harbinense YUAN-3为代表的产氢菌群的优势度显著增强,但丙酸发酵菌属Propionicimonas sp. F6也开始富集。

关键词: 厌氧接触式反应器, 发酵制氢, 进水COD浓度, 菌群结构

Abstract:

The operation performance of fermentative hydrogen production in anaerobic contact reactor (ACR) at different influent COD concentrations was investigated. The ACR could be kept at steady-state with ethanol-type fermentation, as influent chemical oxygen demand (COD) increased from 7000 mg·L^-1to 11000 mg·L^-1 with constant HRT of 6 h. Specific hydrogen production of the ACR system increased from 2.43 m³·(m³·d)^-1to 3.51 m³·(m³·d)^-1 as influent COD increased from 7000 mg·L^-1 to 11000 mg·L^-1, while specific hydrogen production of activated sludge peaked at 10.71 mol H₂·(kg VSS·d)^-1 at influent COD of 9000 mg·L^-1. The results of polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) profiles showed that hydrogen-producing ethanol fermentative bacteria were dominant in the ACR. As influent COD concentration increased, Ethanoligenens harbinense YUAN-3 became more abundant, and propionate fermentative bacteria, i.e. Propionicimonas sp. F6 also started to be enriched.

Key words: anaerobic contact reactor, fermentative hydrogen production, influent COD concentration, microbial community

中图分类号:

昌盛, 李建政, 付青, 赵兴茹, 郑国臣. ACR在不同进水COD浓度下的产氢性能与菌群结构[J]. 化工学报, 2015, 66(3): 1156-1162.

CHANG Sheng, LI Jianzheng, FU Qing, ZHAO Xingru, ZHENG Guochen. Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations[J]. CIESC Journal, 2015, 66(3): 1156-1162.

参考文献 20

[1]	Karapinar I, Kapdan K F. Bio-hydrogen production from waste materials [J]. Enzyme and Microbial Technology, 2006, 38(5): 569-582
[2]	Das D, Veziroglu T N. Advances in biological hydrogen production processes [J]. International Journal of Hydrogen Energy, 2008, 33(21): 6046-6057
[3]	Hawkes F R, Dinsdale R, Hawkes D L, Hussy I. Sustainable fermentative hydrogen production: challenges for process optimization [J]. International Journal of Hydrogen Energy, 2002, 27(11/12): 1339-1347
[4]	David B L, Lawrence P, Murray L. Biohydrogen production: prospects and limitations to practical application [J]. International Journal of Hydrogen Energy, 2004, 29(2): 173-185
[5]	Pakarinen O, Kaparaju P, Rintala J. The effect of organic loading rate and retention time on hydrogen production from a methanogenic CSTR [J]. Bioresource Technology, 2011, 102(19): 8952-8957
[6]	Li Jianzheng(李建政),Yu Ze(于泽), Chang Sheng(昌盛), Su Xiaoyu(苏晓煜). Operation characteristic comparison of CSTR and ACR systems for hydrogen production by butyric acid type fermentation [J]. CIESC Journal(化工学报), 2012, 63(5): 1551-1557
[7]	Puyol D, Mohedano A F, Sanz J L, RodríguezJ J. Comparison of UASB and EGSB performance on the anaerobic biodegradation of 2, 4-dichlorophenol [J]. Chemosphere, 2009, 76(9): 1192-1198
[8]	Kotsopoulos T A, Zeng R J, Angelidaki I. Biohydrogen production in granular up-flow anaerobic sludge blanket (UASB) reactors with mixed cultures under hyper-thermophilic temperature (70℃) [J]. Biotechnology and Bioengineering, 2006, 94(2): 296-302
[9]	Chang J S, Lee K S, Lin P J. Biohydrogen production with fixed-bed bioreactors [J]. International Journal of Hydrogen Energy, 2002, 27: 1167-1174
[10]	Li Jianzheng(李建政), Zhang Ni(张妮), Li Nan(李楠), Wang Xingzu(王兴祖). Influence of HRT on fermentative hydrogen production process by anaerobic activated sludge [J]. Journal of Harbin Institute of Technology(哈尔滨工业大学学报), 2006, 38(11): 1840-1846
[11]	Zhang Z P, Tay J H, Show K Y, Yan R, Liang D T, Lee D J. Biohydrogen production in a granular activated carbon anaerobic fluidized bed reactor [J]. International Journal of Hydrogen Energy, 2007, 32: 185-191
[12]	Guo Wanqian, Ren Nanqi, Wang Xiangjing. Biohydrogen production from ethanol-type fermentation of molasses in an expanded granular sludge bed (EGSB) reactor [J].International Journal of Hydrogen Energy, 2008, 33(19): 4981-4988
[13]	Li Jianzheng(李建政), Li Weiguang(李伟光), Chang Sheng(昌盛), Liu Feng(刘枫), Wang Shujing(王淑静),Zheng Guochen(郑国臣). Start-up and performance of anaerobic contact reactor for hydrogen production [J]. Science & Technology Review(科技导报), 2009, 27(14): 90-93
[14]	Wang Bo, Wan Wei, Wang Jianlong. Inhibitory effect of ethanol, acetic acid, propionic acid and butyric acid on fermentative hydrogen production [J]. International Journal of Hydrogen Energy, 2008, 33(23): 7013-7019
[15]	Ren Nanqi, Li Jianzheng, Li Baikun, Wang Yong, Liu Shirui. Biohydrogen production from molasses by anaerobic fermentation with a pilot-scale bioreactor system [J]. International Journal of Hydrogen Energy, 2006, 31(15): 2147-2157
[16]	Ren Nanqi, Xing Defeng, Rittmann Bruce. Microbial community structure of ethanol type fermentation in bio-hydrogen production [J]. Environmental Microbiology, 2007, 9(5): 1112-1125
[17]	Li Jianzheng(李建政), Chang Sheng(昌盛). Anaerobic contact digester reactor [P]: CN, 200710144460. 2008-05-14
[18]	American Public Health Association. Standard Methods for the Examination of Water and Wastewater [S]. 19th ed. Washington DC, USA, 1998
[19]	Hwang M H, Jang N J, Hyun S H.Anaerobic bio-hydrogen production from ethanol fermentation: the role of pH [J]. Journal Biotechnology, 2004, 111(3): 297-309
[20]	Ren N Q, Wang B Z, Huang J C. Ethanol-type fermentation from carbohydrate in high rate acidogenic reactor [J]. Biotechnol. Bioengineering, 1997, 54(5): 428-433

ACR在不同进水COD浓度下的产氢性能与菌群结构

Performance of fermentative hydrogen production and microbial community in ACR at different influent COD concentrations

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