Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media

CIESC Journal

• BIOTECHNOLOGY & BIOENGINEERING • 上一篇下一篇

Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media

YINChunhua(尹春华),LIUTao(刘涛)andTANTianwei(谭天伟)

College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
School of Applied Science, University of Science and Technology Beijing, Beijing 100083, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-02-28 发布日期:2006-02-28
通讯作者: TAN Tianwei(谭天伟)

Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media

Received:1900-01-01 Revised:1900-01-01 Online:2006-02-28 Published:2006-02-28

摘要/Abstract

摘要： Vitamin A ester was synthesized in organic solvents with immobilized lipase from Candida sp. The types of lipases, influences of solvent, the molar ratio of substrates, the reaction temperature and the water activity in the reaction were studied in detail in order to obtain the optimum conditions for Vitamin A palmitate synthesis. In a system of hexane, 100mg immobilized Candida sp. lipase was used in the presence of 1.2mmol vitamin A acetate and 3.6mmol palmitic acid. The yield of vitamin A palmitate reached 81% in 12h at 25℃. The immobilized Candida sp. lipase was prepared by adsorbing Candida sp. fermentation broth on pretreated textile and could be reused for at least six batches.

关键词: vitamin A;vitamin A palmitate;lipase;immobilization

Key words: vitamin A, vitamin A palmitate, lipase, immobilization

YINChunhua(尹春华),LIUTao(刘涛)andTANTianwei(谭天伟). Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media[J]. CIESC Journal.

YINChunhua(尹春华),LIUTao(刘涛)andTANTianwei(谭天伟). Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media[J]. .

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Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media

Synthesis of Vitamin A Esters by Immobilized Candida sp. Lipase in Organic Media

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