Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R-(－)-Mandelic Acid by Saccharomyces Cerevisiae FD11b

CIESC Journal

• BIOTECHNOLOGY & BIOENGINEERING • 上一篇下一篇

Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R-(－)-Mandelic Acid by Saccharomyces Cerevisiae FD11b

XIAOMeitian(肖美添),HUANGYayan(黄雅燕),MENGChun(孟春)andGUOYanghao(郭养浩)

Institute of Pharmaceutical Biotechnology and Engineering, Fuzhou University, Fuzhou 350001, China
Institute of Pharmaceutical Engineering, Huaqiao University, Quanzhou 362021, China

收稿日期:1900-01-01 修回日期:1900-01-01 出版日期:2006-02-28 发布日期:2006-02-28
通讯作者: XIAO Meitian(肖美添)
XIAO Meitian(肖美添)

Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R-(－)-Mandelic Acid by Saccharomyces Cerevisiae FD11b

Received:1900-01-01 Revised:1900-01-01 Online:2006-02-28 Published:2006-02-28

摘要/Abstract

摘要： The kinetics of asymmetric production of R-(－)-mandelic acid（R-MA）from phenylglyoxylic acid (PGA) catalyzed by Saccharomyces cerevisiae sp. strain FD11b was studied by fed-batch cultures. The concentrations of glucose and PGA were controlled respectively with a dual feeding system. When the electron donor glucose was supplied at the rate of 0.0833mmol?gdw－1?h－1, the specific production rate (qp) and the enantiomeric excess of R-MA reached the maxi-mum 0.353mmol?gdw－1?h－1 and 97.1%, respectively. The apparent reduction activity of yeast FD11b was obviously af-fected by both substrate PGA and product MA. The qp value reached the maximum 0.36—0.38mmol?gdw－1?h－1 when the PGA concentration was controlled between 25 and 35mmol?L－1. The obvious substrate inhibition of bioconversion was observed at the PGA concentrations higher than 40mmol?L－1. The accumulation of product MA also caused a severe feed-back inhibition for its production when the product concentration was above 60mmol?L－1. The kinetic model with the inhibition effect of both substrate and product was simulated by a computer-based least-square arithmatic. The estab-lished kinetic model was in good agreement with the experimental data.

关键词: Saccharomyces cerevisiae;asymmetric reduction;R-(－)-mandelic acid;substrate inhibition;product inhibi-tion;kinetic model

Key words: Saccharomyces cerevisiae, asymmetric reduction, R-(－)-mandelic acid, substrate inhibition, product inhibi-tion, kinetic model

XIAOMeitian(肖美添),HUANGYayan(黄雅燕),MENGChun(孟春)andGUOYanghao(郭养浩). Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R-(－)-Mandelic Acid by Saccharomyces Cerevisiae FD11b[J]. CIESC Journal.

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Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R-(－)-Mandelic Acid by Saccharomyces Cerevisiae FD11b

Kinetics of Asymmetric Reduction of Phenylglyoxylic Acid to R-(－)-Mandelic Acid by Saccharomyces Cerevisiae FD11b

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