化工学报 ›› 2011, Vol. 62 ›› Issue (2): 444-451.
刘国强，金虎，高敏杰，戴科科，汪汇慧，李震，史仲平
LIU Guoqiang，JIN Hu，GAO Minjie，DAI Keke，WANG Huihui，LI Zhen，SHI Zhongping
摘要：
在10 L发酵罐、控制甲醇浓度的条件下，考察了诱导温度对毕赤酵母发酵生产猪α-干扰素（pIFN-α）性能的影响。结果表明，温度对pIFN-α表达的影响明显，20℃低温有利于pIFN-α的表达，该条件下pIFN-α最高活性可达1.5×106 IU·ml-1，是30℃传统诱导生产时最高活性（1.0
关键词:
毕赤酵母；猪α
Abstract:
The effect of induction temperatures on porcine interferon-α(pIFN-α）production byPichia pastoris was investigated in a 10 L bioreactor with methanol concentration controlled at a specific level. The results indicated that a low temperature was obviously beneficial for pIFN-α expression, and the highest pIFN-α antiviral activity reached a level of 1.5×106 IU·ml-1 when induction was made at 20℃, that was 150-folds of the maximal value obtained with the traditional induction strategy at 30℃.On-line multi-variable measurement of the fermentative state variables and metabolic analysis showed that cell metabolic activity and specific methanol consumption rate (0.036 h-1）could be higher with induction at 20
Key words:
毕赤酵母；猪α
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